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Literature summary extracted from

  • Saavedra, C.P.; Encinas, M.V.; Araya, M.A.; Perez, J.M.; Tantalean, J.C.; Fuentes, D.E.; Calderon, I.L.; Pichuantes, S.E.; Vasquez, C.C.
    Biochemical characterization of a thermostable cysteine synthase from Geobacillus stearothermophilus V (2004), Biochimie, 86, 481-485.
    View publication on PubMed

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.5.1.47 0.12
-
 5-thio-2-nitrobenzoate pH 7.0, 50°C Geobacillus stearothermophilus
2.5.1.47 0.36
-
 O3-acetyl-L-serine pH 7.0, 50°C Geobacillus stearothermophilus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.5.1.47 32000
-
 2 * 32000, SDS-PAGE Geobacillus stearothermophilus
2.5.1.47 65000
-
 gel filtration Geobacillus stearothermophilus

Organism

EC Number Organism UniProt Comment Textmining
2.5.1.47 Geobacillus stearothermophilus
-
 overexpression in Escherichia coli
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.5.1.47 beta-chloro-L-alanine + 2-nitro-5-thiobenzoate
-
 Geobacillus stearothermophilus ?
-
 ?
2.5.1.47 additional information no substrate: L-serine, O-propionyl-L-serine, L-alanine, glycine Geobacillus stearothermophilus ?
-
 ?
2.5.1.47 O3-acetyl-L-serine
-
 Geobacillus stearothermophilus alpha-aminoacrylate in absence of S2- and at 50°C, not below ?
2.5.1.47 O3-acetyl-L-serine + 2-nitro-5-thiobenzoate
-
 Geobacillus stearothermophilus ?
-
 ?

Subunits

EC Number Subunits Comment Organism
2.5.1.47 dimer 2 * 32000, SDS-PAGE Geobacillus stearothermophilus

Synonyms

EC Number Synonyms Comment Organism
2.5.1.47 cysK
-
 Geobacillus stearothermophilus

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.5.1.47 154
-
 beta-chloro-L-alanine pH 7.0, 50°C Geobacillus stearothermophilus
2.5.1.47 312
-
 O3-acetyl-L-serine pH 7.0, 50°C Geobacillus stearothermophilus