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Literature summary extracted from

  • Astroem, A.; Eriksson, M.; Eriksson, L.C.; Birberg, W.; Pilotti, A.; DePierre, J.W.
    Subcellular and organ distribution of cholesterol epoxide hydrolase in the rat (1986), Biochim. Biophys. Acta, 882, 359-366.
    View publication on PubMed

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.3.2.9 Golgi apparatus
-
 Rattus norvegicus 5794
-
3.3.2.9 microsome slightly higher enzyme activity in smooth compared to rough microsomes Rattus norvegicus
-
-
3.3.2.9 additional information subcellular distribution analysis Rattus norvegicus
-
-
3.3.2.11 Golgi apparatus
-
 Rattus norvegicus 5794
-
3.3.2.11 microsome similar enzyme activity in smooth and rough microsomes Rattus norvegicus
-
-
3.3.2.11 additional information subcellular distribution analysis, no or low activity in cytosol, plasma membrane, Golgi apparatus II, and nucleus, overview Rattus norvegicus
-
-

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.3.2.9 cis-stilbene oxide + H2O Rattus norvegicus
-
 (+)-(1R,2R)-1,2-diphenylethane-1,2-diol
-
 ?
3.3.2.11 5,6alpha-epoxy-5alpha-cholestan-3beta-ol + H2O Rattus norvegicus
-
 cholestane-3beta,5alpha,6beta-triol
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.3.2.9 Rattus norvegicus
-
 male sprague-dawley rats
-
3.3.2.11 Rattus norvegicus
-
 male sprague-dawley rats
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.3.2.11 partially by subcellular fractionation Rattus norvegicus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.3.2.9 brain very low activity Rattus norvegicus
-
3.3.2.9 intestine epithelium, very low activity Rattus norvegicus
-
3.3.2.9 kidney low activity Rattus norvegicus
-
3.3.2.9 liver highest enzyme activity, specific for Rattus norvegicus
-
3.3.2.9 lung low activity Rattus norvegicus
-
3.3.2.9 additional information tissue distribution analysis Rattus norvegicus
-
3.3.2.9 spleen very low activity Rattus norvegicus
-
3.3.2.9 testis low activity Rattus norvegicus
-
3.3.2.11 brain low activity Rattus norvegicus
-
3.3.2.11 intestine epithelium, low activity Rattus norvegicus
-
3.3.2.11 kidney low activity Rattus norvegicus
-
3.3.2.11 liver highest enzyme activity Rattus norvegicus
-
3.3.2.11 additional information tissue distribution analysis, no or poor activity in lung and spleen Rattus norvegicus
-
3.3.2.11 testis low activity Rattus norvegicus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.3.2.11 additional information
-
 specific activity in different tissues and subcellular fractions Rattus norvegicus
3.3.2.11 0.00018
-
 Golgi apparatus I Rattus norvegicus
3.3.2.11 0.00065
-
 smooth microsomes Rattus norvegicus
3.3.2.11 0.00068
-
 rough microsomes Rattus norvegicus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.3.2.9 cis-stilbene oxide + H2O
-
 Rattus norvegicus (+)-(1R,2R)-1,2-diphenylethane-1,2-diol
-
 ?
3.3.2.11 5,6alpha-epoxy-5alpha-cholestan-3beta-ol + H2O
-
 Rattus norvegicus cholestane-3beta,5alpha,6beta-triol
-
 ?
3.3.2.11 5,6alpha-epoxy-5alpha-cholestan-3beta-ol + H2O the enzyme is highly substrate specific Rattus norvegicus cholestane-3beta,5alpha,6beta-triol
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.3.2.11 cholEH
-
 Rattus norvegicus
3.3.2.11 cholesterol epoxide hydrolase
-
 Rattus norvegicus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.3.2.9 37
-
 assay at Rattus norvegicus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.3.2.9 7
-
 assay at Rattus norvegicus