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Literature summary extracted from

  • Vickaryous, N.K.; Teh, E.M.; Stewart, B.; Dolphin, P.J.; Too, C.K.; McLeod, R.S.
    Deletion of N-terminal amino acids from human lecithin:cholesterol acyltransferase differentially affects enzyme activity toward alpha- and beta-substrate lipoproteins (2003), Biochim. Biophys. Acta, 1646, 164-172.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.3.1.43 apolipoprotein A-I
-
 Homo sapiens

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.3.1.43 expression of wild-type enzyme and N-terminally truncated enzymes in COS-7 cells, secretion to the cell culture Homo sapiens

Protein Variants

EC Number Protein Variants Comment Organism
2.3.1.43 additional information deletion of the first residue of the enzyme's N-terminus lead to 95% reduced alpha-enzyme activity and slightly increased beta-enzyme activity, deletion of the first 2 residues lead to abolished alpha-enzyme activity and reduced beta-enzyme activity, respectively, but the mutant enzymes are still able to bind the substrates, mutants with deletion of 3-5 N-terminal residues show no alpha-enzyme activity and residual or no beta-enzyme activity, overview Homo sapiens

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.3.1.43 extracellular
-
 Homo sapiens
-
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.3.1.43 67000
-
 x * 67000, about, recombinant fully glycosylated wild-type enzyme, SDS-PAGE Homo sapiens

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.3.1.43 phosphatidylcholine + cholesterol Homo sapiens esterification of plasma cholesterol in HDL via alpha-enzyme activity, and of plasma cholesterol in LDL via beta-enzyme activity 1-acylglycerophosphocholine + cholesteryl ester
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.3.1.43 Homo sapiens
-
-
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
2.3.1.43 glycoprotein
-
 Homo sapiens

Reaction

EC Number Reaction Comment Organism Reaction ID
2.3.1.43 phosphatidylcholine + a sterol = 1-acylglycerophosphocholine + a sterol ester structure-function analysis via 3D-modeling of the core and catalytic site of the enzyme Homo sapiens
a

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.3.1.43 plasma
-
 Homo sapiens
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.1.43 additional information substrate binding study of wild-type and mutant enzymes Homo sapiens ?
-
 ?
2.3.1.43 phosphatidylcholine + cholesterol
-
 Homo sapiens 1-acylglycerophosphocholine + cholesteryl ester
-
 ?
2.3.1.43 phosphatidylcholine + cholesterol esterification of plasma cholesterol in HDL via alpha-enzyme activity, and of plasma cholesterol in LDL via beta-enzyme activity Homo sapiens 1-acylglycerophosphocholine + cholesteryl ester
-
 ?

Subunits

EC Number Subunits Comment Organism
2.3.1.43 ? x * 67000, about, recombinant fully glycosylated wild-type enzyme, SDS-PAGE Homo sapiens

Synonyms

EC Number Synonyms Comment Organism
2.3.1.43 LCAT
-
 Homo sapiens
2.3.1.43 lecithin:cholesterol acyltransferase
-
 Homo sapiens

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.3.1.43 37
-
 assay at Homo sapiens

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.3.1.43 8
-
 assay at Homo sapiens