EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
5.1.2.2 | F52W | compared to wild-type enzyme the catalytic preference of the mutant enzyme is reversed and catalytic efficiency is reduced. Mutant enzyme exhibits higher affinity for (R)-mandelate than for (S)-mandelate, and a higher turnover number with (S)-mandelate as the substrate, relative to that with (R)-mandelate | Pseudomonas putida |
5.1.2.2 | F52W/Y54W | compared to wild-type enzyme the catalytic preference of the mutant enzyme is reversed and catalytic efficiency is reduced. Mutant enzyme exhibits higher affinity for (R)-mandelate than for (S)-mandelate, and a higher turnover number with (S)-mandelate as the substrate, relative to that with (R)-mandelate | Pseudomonas putida |
5.1.2.2 | Y54Q | compared to wild-type enzyme the catalytic preference of the mutant enzyme is reversed and catalytic efficiency is reduced. Mutant enzyme exhibits higher affinity for (R)-mandelate than for (S)-mandelate, and a higher turnover number with (S)-mandelate as the substrate, relative to that with (R)-mandelate | Pseudomonas putida |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
5.1.2.2 | (R)-2-hydroxybutyrate | - |
Pseudomonas putida | |
5.1.2.2 | (R)-2-naphthylglycolate | - |
Pseudomonas putida | |
5.1.2.2 | (S)-2-hydroxybutyrate | - |
Pseudomonas putida | |
5.1.2.2 | (S)-2-naphthylglycolate | - |
Pseudomonas putida | |
5.1.2.2 | (S)-cyclohexylphenylglycolate | - |
Pseudomonas putida | |
5.1.2.2 | benzilate | - |
Pseudomonas putida | |
5.1.2.2 | diphenylacetate | - |
Pseudomonas putida |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
5.1.2.2 | 0.29 | - |
(R)-mandelate | mutant enzyme F52W/Y64W | Pseudomonas putida | |
5.1.2.2 | 0.35 | - |
(R)-2-naphthylglycolate | mutant enzyme F52W/Y54W | Pseudomonas putida | |
5.1.2.2 | 0.41 | - |
(S)-2-naphthylglycolate | wild-type enzyme | Pseudomonas putida | |
5.1.2.2 | 0.46 | - |
(R)-2-naphthylglycolate | wild-type enzyme | Pseudomonas putida | |
5.1.2.2 | 0.54 | - |
(S)-Mandelate | wild-type enzyme | Pseudomonas putida | |
5.1.2.2 | 0.7 | - |
(R)-mandelate | wild-type enzyme | Pseudomonas putida | |
5.1.2.2 | 1.1 | - |
(R)-mandelate | mutant enzyme Y54W | Pseudomonas putida | |
5.1.2.2 | 1.5 | - |
(S)-2-naphthylglycolate | mutant enzyme F52W/Y54W | Pseudomonas putida | |
5.1.2.2 | 2.5 | - |
(R)-mandelate | mutant enzyme F52W | Pseudomonas putida | |
5.1.2.2 | 2.9 | - |
(S)-Mandelate | mutant enzyme Y54W | Pseudomonas putida | |
5.1.2.2 | 3.3 | - |
(S)-Mandelate | mutant enzyme F52W | Pseudomonas putida | |
5.1.2.2 | 3.8 | - |
(S)-Mandelate | mutant enzyme F52W/Y64W | Pseudomonas putida |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
5.1.2.2 | Pseudomonas putida | P11444 | - |
- |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
5.1.2.2 | (R)-2-naphthylglycolate | - |
Pseudomonas putida | (S)-2-naphthylglycolate | - |
r | |
5.1.2.2 | (R)-mandelate | wild-type enzyme shows slightly higher affinity for (S)-mandelate than for (R)-mandelate, but catalyzes the turnover of (R)-mandelate slightly more rapidly | Pseudomonas putida | (S)-mandelate | - |
r |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
5.1.2.2 | 1 | - |
(R)-mandelate | mutant enzyme F52W/Y64W | Pseudomonas putida | |
5.1.2.2 | 2 | 8 | (S)-Mandelate | mutant enzyme Y54W | Pseudomonas putida | |
5.1.2.2 | 10 | - |
(R)-mandelate | mutant enzyme Y54W | Pseudomonas putida | |
5.1.2.2 | 12 | - |
(S)-Mandelate | mutant enzyme F52W/Y64W | Pseudomonas putida | |
5.1.2.2 | 159 | - |
(R)-mandelate | mutant enzyme F52W | Pseudomonas putida | |
5.1.2.2 | 248 | - |
(S)-Mandelate | mutant enzyme F52W | Pseudomonas putida | |
5.1.2.2 | 467 | - |
(S)-Mandelate | wild-type enzyme | Pseudomonas putida | |
5.1.2.2 | 526 | - |
(R)-mandelate | wild-type enzyme | Pseudomonas putida |
EC Number | Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|---|
5.1.2.2 | 0.16 | - |
(R)-2-naphthylglycolate | mutant enzyme F52W/Y54W | Pseudomonas putida | |
5.1.2.2 | 0.48 | - |
(S)-2-naphthylglycolate | mutant enzyme F52W/Y54W | Pseudomonas putida | |
5.1.2.2 | 0.5 | - |
(S)-cyclohexylphenylglycolate | wild-type enzyme | Pseudomonas putida | |
5.1.2.2 | 0.67 | - |
benzilate | wild-type enzyme | Pseudomonas putida | |
5.1.2.2 | 2 | 5 | (S)-2-naphthylglycolate | wild-type enzyme | Pseudomonas putida | |
5.1.2.2 | 4.5 | - |
diphenylacetate | wild-type enzyme | Pseudomonas putida | |
5.1.2.2 | 11.4 | - |
(S)-2-hydroxybutyrate | wild-type enzyme | Pseudomonas putida | |
5.1.2.2 | 17.3 | - |
(R)-2-hydroxybutyrate | wild-type enzyme | Pseudomonas putida | |
5.1.2.2 | 33 | - |
(R)-2-naphthylglycolate | wild-type enzyme | Pseudomonas putida | |
5.1.2.2 | 45 | - |
benzilate | mutant enzyme F52W/Y54W | Pseudomonas putida | |
5.1.2.2 | 71 | - |
(S)-2-hydroxybutyrate | mutant enzyme F52W/Y54W | Pseudomonas putida | |
5.1.2.2 | 98 | - |
(R)-2-hydroxybutyrate | mutant enzyme F52W/Y54W | Pseudomonas putida |