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Literature summary extracted from

  • Claus, M.T.; Zocher, G.E.; Maier, T.H.; Schulz, G.E.
    Structure of the O-acetylserine sulfhydrylase isoenzyme CysM from Escherichia coli (2005), Biochemistry, 44, 8620-8626.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.5.1.47 expressed in Escherichia coli BL21(DE3) cells Escherichia coli

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
2.5.1.47
-
 Escherichia coli
2.5.1.47 hanging drop vapor diffusion method, using 100 mM sodium citrate, pH 5.6, 100 mM ammonium sulfate, 20% (w/v) PEG 4000 Escherichia coli

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.5.1.47 32893
-
 2 * 32893, calculated from amino acid sequence Escherichia coli

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.5.1.47 O-acetyl-L-serine + hydrogen sulfide Escherichia coli
-
 L-cysteine + acetate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.5.1.47 Escherichia coli P16703
-
-
2.5.1.47 Escherichia coli P16703 isoenzyme CysM
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.5.1.47 Source 30Q column chromatography and Superdex 75 gel filtration Escherichia coli

Reaction

EC Number Reaction Comment Organism Reaction ID
2.5.1.47 O-acetyl-L-serine + hydrogen sulfide = L-cysteine + acetate geometric model of reaction, comparison isoenzyme CysK Escherichia coli
a

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.5.1.47 O-acetyl-L-serine + hydrogen sulfide
-
 Escherichia coli L-cysteine + acetate
-
 ?

Subunits

EC Number Subunits Comment Organism
2.5.1.47 homodimer 2 * 32893, calculated from amino acid sequence Escherichia coli

Synonyms

EC Number Synonyms Comment Organism
2.5.1.47 CysM
-
 Escherichia coli
2.5.1.47 O-acetylserine sulfhydrylase isoenzyme B
-
 Escherichia coli

Cofactor

EC Number Cofactor Comment Organism Structure
2.5.1.47 pyridoxal 5'-phosphate
-
 Escherichia coli