EC Number | Activating Compound | Comment | Organism | Structure |
---|---|---|---|---|
6.1.1.10 | additional information | bovine serum albumin has no effect on the enzyme | Homo sapiens | |
6.1.1.10 | spermine | slight stimulation, additionally spermine increases the enzyme activity by stabilizing conformation of negatively charged DNA | Homo sapiens |
EC Number | Cloned (Comment) | Organism |
---|---|---|
6.1.1.10 | mature enzyme form, DNA and amino acid sequence determination and analysis, expression of His-tagged enzyme in Escherichia coli | Homo sapiens |
EC Number | General Stability | Organism |
---|---|---|
6.1.1.10 | bovine serum albumin has no effect on the enzyme | Homo sapiens |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
6.1.1.10 | 0.00015 | - |
tRNAMet | pH 8.2, 37°C, mitochondrial tRNAMet from Bos taurus, recombinant enzyme | Homo sapiens | |
6.1.1.10 | 0.00016 | - |
tRNAMet | pH 8.2, 37°C, human mitochondrial wild-type tRNAMet, recombinant enzyme | Homo sapiens | |
6.1.1.10 | 0.0021 | - |
tRNAMet | pH 8.2, 37°C, initiator tRNA from Escherichia coli, recombinant enzyme | Homo sapiens | |
6.1.1.10 | 0.018 | - |
L-methionine | pH 8.2, 37°C, recombinant enzyme | Homo sapiens | |
6.1.1.10 | 0.085 | - |
ATP | pH 8.2, 37°C, recombinant enzyme | Homo sapiens |
EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|---|
6.1.1.10 | mitochondrion | mtMetRS | Homo sapiens | 5739 | - |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
6.1.1.10 | KCl | stimulates | Homo sapiens | |
6.1.1.10 | Mg2+ | - |
Homo sapiens |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
6.1.1.10 | 67000 | - |
gel filtration | Homo sapiens |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
6.1.1.10 | ATP + L-methionine + tRNAMet | Homo sapiens | - |
AMP + diphosphate + L-methionyl-tRNAMet | - |
r |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
6.1.1.10 | Homo sapiens | P56192 | class I enzyme | - |
EC Number | Purification (Comment) | Organism |
---|---|---|
6.1.1.10 | recombinant His-tagged mature enzyme form from Escherichia coli by ion exchange chromatography | Homo sapiens |
EC Number | Reaction | Comment | Organism | Reaction ID |
---|---|---|---|---|
6.1.1.10 | ATP + L-methionine + tRNAMet = AMP + diphosphate + L-methionyl-tRNAMet | two-step reaction process via anenzyme-L-Met-AMP intermediate | Homo sapiens |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
6.1.1.10 | ATP + L-methionine + tRNAMet | - |
Homo sapiens | AMP + diphosphate + L-methionyl-tRNAMet | - |
r | |
6.1.1.10 | ATP + L-methionine + tRNAMet | human mitochondrial wild-type and mutant mtRNAs: T5048 deletion, and T5052C or T5012A point mutations, initiator tRNA from Escherichia coli, tRNAMet from Bos taurus | Homo sapiens | AMP + diphosphate + L-methionyl-tRNAMet | - |
r | |
6.1.1.10 | additional information | the enzyme performs ATP/diphosphate exchange in absence of substrates | Homo sapiens | ? | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
6.1.1.10 | monomer | 1 * 67000, SDS-PAGE | Homo sapiens |
6.1.1.10 | More | domain structure, structure modeling based on crystal structures of Escherichia coli with PDB code 1QQR, overview, the enzyme contains the consensus motifs of class I aminoacyl-tRNA, but lacks the Zn2+ binding motif and the C-terminal dimerization region | Homo sapiens |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
6.1.1.10 | methionyl-tRNA synthetase | - |
Homo sapiens |
6.1.1.10 | MetRS | - |
Homo sapiens |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
6.1.1.10 | 37 | - |
assay at | Homo sapiens |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
6.1.1.10 | 0.019 | - |
tRNAMet | pH 8.2, 37°C, mitochondrial tRNAMet from Bos taurus, recombinant enzyme | Homo sapiens | |
6.1.1.10 | 0.021 | - |
tRNAMet | pH 8.2, 37°C, human mitochondrial wild-type tRNAMet, recombinant enzyme | Homo sapiens | |
6.1.1.10 | 0.033 | - |
ATP | pH 8.2, 37°C, recombinant enzyme | Homo sapiens | |
6.1.1.10 | 0.41 | - |
L-methionine | pH 8.2, 37°C, recombinant enzyme | Homo sapiens | |
6.1.1.10 | 11 | - |
tRNAMet | pH 8.2, 37°C, initiator tRNA from Escherichia coli, recombinant enzyme | Homo sapiens |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
6.1.1.10 | 7.8 | 8.2 | - |
Homo sapiens |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
6.1.1.10 | ATP | - |
Homo sapiens |