Literature summary extracted from

Wu, J.; Jones, J.M.; Nguyen-Huu, X.; Ten Eyck, L.F.; Taylor, S.S.
Crystal structures of RIalpha subunit of cyclic adenosine 5'-monophosphate (cAMP)-dependent protein kinase complexed with (Rp)-adenosine 3',5'-cyclic monophosphothioate and (Sp)-adenosine 3',5'-cyclic monophosphothioate, the phosphothioate analogues of cAMP (2004), Biochemistry, 43, 6620-6629.

Activating Compound

EC Number	Activating Compound	Comment	Organism	Structure
2.7.11.11	cAMP	dependent on, binding site	Bos taurus

Cloned(Commentary)

EC Number	Cloned (Comment)	Organism
2.7.11.11	expression of regulatory subunit deletion mutant RIalpha comprising residues 1-91	Bos taurus

Crystallization (Commentary)

EC Number	Crystallization (Comment)	Organism
2.7.11.11	purified recombinant regulatory subunit deletion mutant RIalpha bound to cAMP analogue ligands (Rp)-cAMPS or (Sp)-cAMPS resulting in (Rp)-RIalpha and (Sp)-RIalpha, hanging drop vapour diffusion method, mixing of 0.002ml protein solution containing 12 mg/ml protein in 50 mM MES, pH 7.5, 0.2 M NaCl, 2 mM EDTA, 2 mM EGTA, and 10 mM DTT, with 0.002 ml reservoir solution containing 1.0 M ammonium sulfate, 12.5% glycerol, 10 mM DTT, 0.1 M sodium acetate, pH 5.5, 22.5°C, 1 week, X-ray diffraction structure determination and analysis at 2.3-2.4 A resolution	Bos taurus

Inhibitors

EC Number	Inhibitors	Comment	Organism	Structure
2.7.11.11	(Rp)-adenosine 3',5'-cyclic monophosphothioate	i.e. (Rp)-cAMPS, analogue of cAMP, competitive inhibition of PKA activation by cAMP, binding site structure	Bos taurus
2.7.11.11	(Sp)-adenosine 3',5'-cyclic monophosphothioate	i.e. (Sp)-cAMPS, analogue of cAMP, competitive inhibition of PKA activation by cAMP, binding site structure	Bos taurus

Organism

EC Number	Organism	UniProt	Comment	Textmining
2.7.11.11	Bos taurus	-	-	-

Purification (Commentary)

EC Number	Purification (Comment)	Organism
2.7.11.11	recombinant regulatory subunit deletion mutant RIalpha by affinity chromatography, elution with cGMP, and dialysis	Bos taurus

Subunits

EC Number	Subunits	Comment	Organism
2.7.11.11	More	structure of residues 1-91 of the regulatory subunit	Bos taurus

Synonyms

EC Number	Synonyms	Comment	Organism
2.7.11.11	PKA	-	Bos taurus

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Release 2023.1 (January 2023)