EC Number | Cloned (Comment) | Organism |
---|---|---|
6.4.1.1 | expression in Escherichia coli JM109 | Geobacillus thermodenitrificans |
EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
6.4.1.1 | D543E | mutant with decreased activity | Geobacillus thermodenitrificans |
6.4.1.1 | D649N | mutant with decreased activity | Geobacillus thermodenitrificans |
6.4.1.1 | D713E | mutant with decreased activity | Geobacillus thermodenitrificans |
6.4.1.1 | D713N | mutant with decreased activity | Geobacillus thermodenitrificans |
6.4.1.1 | D762E | mutant with decreased activity | Geobacillus thermodenitrificans |
6.4.1.1 | D762N | mutant with decreased activity | Geobacillus thermodenitrificans |
6.4.1.1 | E576D | mutant with decreased activity | Geobacillus thermodenitrificans |
6.4.1.1 | E576Q | mutant with decreased activity | Geobacillus thermodenitrificans |
6.4.1.1 | E592Q | mutant with decreased activity | Geobacillus thermodenitrificans |
6.4.1.1 | K712Q | mutant with decreased activity | Geobacillus thermodenitrificans |
6.4.1.1 | K712R | mutant with decreased activity | Geobacillus thermodenitrificans |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
6.4.1.1 | 0.28 | - |
pyruvate | D762N | Geobacillus thermodenitrificans | |
6.4.1.1 | 0.29 | - |
pyruvate | D762E | Geobacillus thermodenitrificans | |
6.4.1.1 | 0.3 | - |
pyruvate | wild type | Geobacillus thermodenitrificans | |
6.4.1.1 | 0.41 | - |
pyruvate | K712R | Geobacillus thermodenitrificans | |
6.4.1.1 | 0.44 | - |
pyruvate | E576D | Geobacillus thermodenitrificans | |
6.4.1.1 | 0.49 | - |
pyruvate | E592Q | Geobacillus thermodenitrificans | |
6.4.1.1 | 0.58 | - |
pyruvate | D713E | Geobacillus thermodenitrificans | |
6.4.1.1 | 1.16 | - |
pyruvate | D713N | Geobacillus thermodenitrificans | |
6.4.1.1 | 1.2 | - |
pyruvate | D649N | Geobacillus thermodenitrificans | |
6.4.1.1 | 1.62 | - |
pyruvate | E576Q | Geobacillus thermodenitrificans | |
6.4.1.1 | 16.2 | - |
pyruvate | D543E | Geobacillus thermodenitrificans |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
6.4.1.1 | ATP + pyruvate + HCO3- | Geobacillus thermodenitrificans | - |
ADP + oxaloacetate + phosphate | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
6.4.1.1 | Geobacillus thermodenitrificans | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
6.4.1.1 | - |
Geobacillus thermodenitrificans |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
6.4.1.1 | ATP + pyruvate + HCO3- | - |
Geobacillus thermodenitrificans | ADP + oxaloacetate + phosphate | - |
? |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
6.4.1.1 | biotin | - |
Geobacillus thermodenitrificans |