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Literature summary extracted from

  • Hollinger, S.; Ramineni, S.; Hepler, J.R.
    Phosphorylation of RGS14 by protein kinase A potentiates its activity toward Galphai (2003), Biochemistry, 42, 811-819.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.7.11.11 cAMP dependent on, stimulates phosphorylation of RGS protein Rattus norvegicus
2.7.11.11 additional information forskolin stimulates the adenylyl cyclase, which activates the enzyme by increased cAMP level Rattus norvegicus

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.11.11 H89 specific inhibition Rattus norvegicus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.11.11 Mg2+
-
 Rattus norvegicus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.11.11 ATP + RGS protein Rattus norvegicus recombinant HA-tagged substrate expressed in B35 cells, phosphorylation of RGS14 by PKA potentiates its activity toward Galphai-GDP ADP + RGS protein phosphate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.7.11.11 Rattus norvegicus
-
-
-

Reaction

EC Number Reaction Comment Organism Reaction ID
2.7.11.11 ATP + a [protein]-(L-serine/L-threonine) = ADP + a [protein]-(L-serine/L-threonine) phosphate the substrate binds to the regulatory subunit which is released from the catalytic subunit for enzyme activity, mechanism and structure-function relationship Rattus norvegicus
a

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.7.11.11 B35 cell neuroblastoma cell line Rattus norvegicus
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.11.11 ATP + RGS protein recombinant HA-tagged substrate expressed in B35 cells, phosphorylation of RGS14 by PKA potentiates its activity toward Galphai-GDP Rattus norvegicus ADP + RGS protein phosphate
-
 ?
2.7.11.11 ATP + RGS protein i.e. regulator of G protein signaling protein, recombinant His-tagged substrate R14-RSG residues 1-205 and 299-544, phosphorylation of wild-type R14-RSG at Ser258 and Thr494 Rattus norvegicus ADP + RGS protein phosphate
-
 ?

Subunits

EC Number Subunits Comment Organism
2.7.11.11 More the substrate binds to the regulatory subunit which is released from the catalytic subunit for enzyme activity Rattus norvegicus

Synonyms

EC Number Synonyms Comment Organism
2.7.11.11 PKA
-
 Rattus norvegicus
2.7.11.11 protein kinase A
-
 Rattus norvegicus

Cofactor

EC Number Cofactor Comment Organism Structure
2.7.11.11 ATP
-
 Rattus norvegicus