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Literature summary extracted from

  • Zhao, Y.; Wang, J.; Gebre, A.K.; Chisholm, J.W.; Parks, J.S.
    Negative charge at amino acid 149 is the molecular determinant for substrate specificity of lecithin: cholesterol acyltransferase for phosphatidylcholine containing 20-carbon sn-2 fatty acyl chains (2003), Biochemistry, 42, 13941-13949.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.3.1.43 transient expression of wild-type and mutant enzymes in COS-1 cells Homo sapiens

Protein Variants

EC Number Protein Variants Comment Organism
2.3.1.43 E149A site-directed mutagenesis, mutation alters the human enzyme residue to the corresponding residue of the rat sequence, 2.9fold increased cholesteryl ester formation activity, 5.5fold increased phospholipase A2 activity in the mutant compared to the wild-type enzyme Homo sapiens
2.3.1.43 E149A/Y292H/W294F site-directed mutagenesis, mutation alters the human enzyme residues to the corresponding residues of the rat sequence, increased cholesteryl ester formation activity and phospholipase A2 activity with 1-palmitoyl-2-20:4-sn-glycero-3-phosphocholine, decreased activities with 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine compared to the wild-type enzyme Homo sapiens
2.3.1.43 Y292H/W294F site-directed mutagenesis, mutation alters the human enzyme residues to the corresponding residues of the rat sequence, 1.4fold increased cholesteryl ester formation activity, 2.8fold increased phospholipase A2 activity in the mutant compared to the wild-type enzyme Homo sapiens

Organism

EC Number Organism UniProt Comment Textmining
2.3.1.43 Homo sapiens
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.3.1.43 recombinant wild-type and mutant enzymes from COS-1 cells Homo sapiens

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.3.1.43 additional information
-
 cholesterol esterification and phospholipase A2 activities of wild-type and mutant enzymes with different substrates, overview Homo sapiens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.1.43 1-palmitoyl-2-20:4-sn-glycero-3-phosphocholine + cholesterol
-
 Homo sapiens ?
-
 ?
2.3.1.43 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine + cholesterol
-
 Homo sapiens ?
-
 ?
2.3.1.43 additional information the fatty acid substrate specificity is determined by residues E149, Y292, and W294, enzyme also shows phospholipase A2 activity with the substrates Homo sapiens ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.3.1.43 LCAT
-
 Homo sapiens
2.3.1.43 lecithin: cholesterol acyltransferase
-
 Homo sapiens