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Literature summary extracted from
- Functional characterization of alpha-glucan,water dikinase, the starch phosphorylating enzyme (2004), Biochem. J., 377, 525-532.
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 2.7.9.4 | expression in Escherichia coli | Solanum tuberosum |
Protein Variants
| EC Number | Protein Variants | Comment | Organism |
|---|---|---|---|
| 2.7.9.4 | H992A | mutant enzyme backgroun level of activity, no autophosphorylation | Solanum tuberosum |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 2.7.9.4 | 330000 | - |
gel filtration | Solanum tuberosum |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 2.7.9.4 | Solanum tuberosum | Q9AWA5 | - |
- |
Posttranslational Modification
| EC Number | Posttranslational Modification | Comment | Organism |
|---|---|---|---|
| 2.7.9.4 | phosphoprotein | autophosphorylation of wild-type enzyme, no histidine autophosphorylation of mutant enzyme H992A | Solanum tuberosum |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 2.7.9.4 | wild-type and mutant enzyme H992A | Solanum tuberosum |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 2.7.9.4 | amylopectin + ATP + H2O | - |
Solanum tuberosum | AMP + phospho-amylopectin + phosphate | - |
? |  |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 2.7.9.4 | dimer | - |
Solanum tuberosum |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 2.7.9.4 | 35 | - |
- |
Solanum tuberosum |
Temperature Range [°C]
| EC Number | Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 2.7.9.4 | 20 | 40 | 20°C: about 75% of maximal activity, 40°C: about 85% of maximal activity | Solanum tuberosum |
Temperature Stability [°C]
| EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 2.7.9.4 | 45 | - |
5 min, about 15% loss of activity | Solanum tuberosum |
| 2.7.9.4 | 50 | - |
5 min, about 40% loss of activity | Solanum tuberosum |
| 2.7.9.4 | 55 | - |
5 min, complete loss of activity | Solanum tuberosum |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 2.7.9.4 | 7 | - |
- |
Solanum tuberosum |
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Release 2023.1 (January 2023)
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