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Literature summary extracted from

  • Wongsantichon, J.; Harnnoi, T.; Ketterman, A.J.
    A sensitive core region in the structure of glutathione S-transferases (2003), Biochem. J., 373, 759-765.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.5.1.18 wild-type and mutant enzymes Anopheles dirus

Protein Variants

EC Number Protein Variants Comment Organism
2.5.1.18 L103E the ratio of turnover number to KM-value for 1-chloro-2,4-dinitrobenzene is 1.8fold higher than wild-type ratio, the ratio of turnover number to KM-value for GSH is 2.9fold lower than wild-type ratio. Half-life of mutant enzyme at 45°C is below 1 min, compared to half-life of wild-type enzyme, which is about 13 min Anopheles dirus
2.5.1.18 L103I the ratio of turnover number to KM-value for 1-chloro-2,4-dinitrobenzene is 3fold higher than wild-type ratio, the ratio of turnover number to KM-value for GSH is 2.7fold lower than wild-type ratio. Half-life of mutant enzyme at 45°C is below 1 min, compared to half-life of wild-type enzyme, which is about 13 min Anopheles dirus
2.5.1.18 L103M the ratio of turnover number to KM-value for 1-chloro-2,4-dinitrobenzene is 3.6fold higher than wild-type ratio, the ratio of turnover number to KM-value for GSH is 5.7fold lower than wild-type ratio. Increased half-life at 45°C as compared to wild-type enzyme Anopheles dirus
2.5.1.18 L103N expressed as insoluble inclusion bodies in Escherichia coli. The ratio of turnover number to KM-value for 1-chloro-2,4-dinitrobenzene is 1.15fold higher than wild-type ratio, the ratio of turnover number to KM-value for GSH is 44.3fold lower than wild-type ratio. Half-life of mutant enzyme at 45°C is below 1 min, compared to half-life of wild-type enzyme, which is about 13 min Anopheles dirus
2.5.1.18 L103R the ratio of turnover number to KM-value for 1-chloro-2,4-dinitrobenzene is fold higher than wild-type ratio, the ratio of turnover number to KM-value for GSH is fold lower than wild-type ratio. Half-life of mutant enzyme at 45°C is below 1 min, compared to half-life of wild-type enzyme, which is about 13 min Anopheles dirus
2.5.1.18 L103Y expressed as insoluble inclusion bodies in Escherichia coli. The ratio of turnover number to KM-value for 1-chloro-2,4-dinitrobenzene is fold higher than wild-type ratio, the ratio of turnover number to KM-value for GSH is fold lower than wild-type ratio. Half-life of mutant enzyme at 45°C is below 1 min, compared to half-life of wild-type enzyme, which is about 13 min Anopheles dirus
2.5.1.18 L104A the ratio of turnover number to KM-value for 1-chloro-2,4-dinitrobenzene is 1.3fold higher than wild-type ratio, the ratio of turnover number to KM-value for GSH is 33.3fold lower than wild-type ratio. Half-life of mutant enzyme at 45°C is below 1 min, compared to half-life of wild-type enzyme, which is about 13 min Anopheles dirus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.5.1.18 0.49
-
 1-chloro-2,4-dinitrobenzene mutant enzyme L103I Anopheles dirus
2.5.1.18 0.62
-
 1-chloro-2,4-dinitrobenzene mutant enzyme L103A Anopheles dirus
2.5.1.18 0.7
-
 GSH wild-type enzyme Anopheles dirus
2.5.1.18 0.72
-
 1-chloro-2,4-dinitrobenzene mutant enzyme L103E Anopheles dirus
2.5.1.18 0.76
-
 1-chloro-2,4-dinitrobenzene wild-type enzyme Anopheles dirus
2.5.1.18 0.77
-
 1-chloro-2,4-dinitrobenzene mutant enzyme L103N Anopheles dirus
2.5.1.18 0.91
-
 1-chloro-2,4-dinitrobenzene mutant enzyme L103M Anopheles dirus
2.5.1.18 3.42
-
 GSH mutant enzyme L103E Anopheles dirus
2.5.1.18 3.7
-
 GSH mutant enzyme L103I Anopheles dirus
2.5.1.18 6.38
-
 GSH mutant enzyme L103M Anopheles dirus
2.5.1.18 24
-
 GSH mutant enzyme L103A Anopheles dirus
2.5.1.18 37.4
-
 GSH mutant enzyme L103N Anopheles dirus

Organism

EC Number Organism UniProt Comment Textmining
2.5.1.18 Anopheles dirus
-
 enzyme form AdGSTD4-4
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.5.1.18
-
 Anopheles dirus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.5.1.18 1-chloro-2,4-dinitrobenzene + glutathione
-
 Anopheles dirus S-(2,4-dinitrophenyl)glutathione + HCl
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.5.1.18 adGSTD4-4
-
 Anopheles dirus
2.5.1.18 glutathione S-transferase
-
 Anopheles dirus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.5.1.18 45
-
 half-life of wild-type enzyme is about 13 min, half-life of mutant enzyme L103M is about 15 min, half-life of mutant enzymes L103N, L103A, L103E and l103I is below 1 min Anopheles dirus

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.5.1.18 18.6
-
 GSH wild-type enzyme Anopheles dirus
2.5.1.18 18.6
-
 1-chloro-2,4-dinitrobenzene wild-type enzyme Anopheles dirus
2.5.1.18 19.78
-
 GSH mutant enzyme L103A Anopheles dirus
2.5.1.18 19.78
-
 1-chloro-2,4-dinitrobenzene mutant enzyme L103A Anopheles dirus
2.5.1.18 21.94
-
 GSH mutant enzyme L103N Anopheles dirus
2.5.1.18 21.94
-
 1-chloro-2,4-dinitrobenzene mutant enzyme L103N Anopheles dirus
2.5.1.18 30.16
-
 GSH mutant enzyme L103M Anopheles dirus
2.5.1.18 30.16
-
 1-chloro-2,4-dinitrobenzene mutant enzyme L103M Anopheles dirus
2.5.1.18 31.9
-
 GSH mutant enzyme L103E Anopheles dirus
2.5.1.18 31.9
-
 1-chloro-2,4-dinitrobenzene mutant enzyme L103E Anopheles dirus
2.5.1.18 36.21
-
 GSH mutant enzyme L103I Anopheles dirus
2.5.1.18 36.21
-
 1-chloro-2,4-dinitrobenzene mutant enzyme L103I Anopheles dirus