EC Number | Activating Compound | Comment | Organism | Structure |
---|---|---|---|---|
1.14.11.26 | ammonium chloride | - |
Acremonium chrysogenum | |
1.14.11.26 | ammonium sulfate | - |
Acremonium chrysogenum | |
1.14.11.26 | ascorbate | required | Acremonium chrysogenum | |
1.14.11.26 | dithiothreitol | up to 30% activation in presence of dithiothreitol | Acremonium chrysogenum | |
1.14.20.1 | ammonium chloride | - |
Acremonium chrysogenum | |
1.14.20.1 | ammonium sulfate | - |
Acremonium chrysogenum | |
1.14.20.1 | ascorbate | required | Acremonium chrysogenum | |
1.14.20.1 | dithiothreitol | up to 30% activation in presence of dithiothreitol | Acremonium chrysogenum |
EC Number | General Stability | Organism |
---|---|---|
1.14.11.26 | sodium phosphate and Bis/Tris buffers are inhibitory | Acremonium chrysogenum |
1.14.20.1 | sodium phosphate and Bis/Tris buffers are inhibitory | Acremonium chrysogenum |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
1.14.11.26 | (2S,4S,6R,7R)-1-aza-7-((5R)-5-carboxypentanamidol)-4-methyl-8-oxo-5-thiatricyclo-(4,2,0,0)octane-2-carboxylate | reversible, 0.04 mM, 90% inhibition | Acremonium chrysogenum | |
1.14.11.26 | ammonium hydrogencarbonate | 100-500 mM, complete inactivation | Acremonium chrysogenum | |
1.14.11.26 | bathophenanthroline | - |
Acremonium chrysogenum | |
1.14.11.26 | N-ethylmaleimide | - |
Acremonium chrysogenum | |
1.14.20.1 | (2S,4S,6R,7R)-1-aza-7-((5R)-5-carboxypentanamidol)-4-methyl-8-oxo-5-thiatricyclo-(4,2,0,0)octane-2-carboxylate | reversible, 0.04 mM, 90% inhibition | Acremonium chrysogenum | |
1.14.20.1 | ammonium hydrogencarbonate | 100-500 mM, complete inactivation | Acremonium chrysogenum | |
1.14.20.1 | bathophenanthroline | - |
Acremonium chrysogenum | |
1.14.20.1 | N-ethylmaleimide | - |
Acremonium chrysogenum |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
1.14.11.26 | 40000 | - |
x * 40000, SDS-PAGE | Acremonium chrysogenum |
1.14.20.1 | 40000 | - |
x * 40000, SDS-PAGE | Acremonium chrysogenum |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.14.11.26 | Acremonium chrysogenum | - |
enzyme catalyzes both reaction of EC 1.14.20.1 and of EC 1.14.11.26 | - |
1.14.20.1 | Acremonium chrysogenum | - |
enzyme catalyzes both reaction of EC 1.14.20.1 and of EC 1.14.11.26 | - |
EC Number | Purification (Comment) | Organism |
---|---|---|
1.14.11.26 | - |
Acremonium chrysogenum |
1.14.20.1 | - |
Acremonium chrysogenum |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
1.14.20.1 | 38.5 | - |
pH 7.4, 27°C | Acremonium chrysogenum |
EC Number | Storage Stability | Organism |
---|---|---|
1.14.11.26 | -70°C, presence of dithiothreitol, stable for 4 weeks | Acremonium chrysogenum |
1.14.11.26 | 4°C, presence of dithiothreitol, half-life is 48 h | Acremonium chrysogenum |
1.14.20.1 | -70°C, presence of dithiothreitol, stable for 4 weeks | Acremonium chrysogenum |
1.14.20.1 | 4°C, presence of dithiothreitol, half-life is 48 h | Acremonium chrysogenum |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.14.11.26 | deacetoxycephalosporin C + 2-oxoglutarate + O2 | - |
Acremonium chrysogenum | deacetylcephalosporin C + succinate + CO2 + H2O | - |
? | |
1.14.20.1 | additional information | no substrate: pyruvate, 2-oxoadipate | Acremonium chrysogenum | ? | - |
? | |
1.14.20.1 | penicillin N + 2-oxoglutarate + O2 | - |
Acremonium chrysogenum | deacetoxycephalosporin C + succinate + CO2 + H2O | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
1.14.11.26 | ? | x * 40000, SDS-PAGE | Acremonium chrysogenum |
1.14.20.1 | ? | x * 40000, SDS-PAGE | Acremonium chrysogenum |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
1.14.11.26 | 7.5 | - |
- |
Acremonium chrysogenum |
1.14.20.1 | 7.5 | - |
- |
Acremonium chrysogenum |