Data extracted from this reference:
Cloned(Commentary)
1.1.1.41
expressed in Escherichia coli strain BL21-CodonPlus (DE3)-RIL
Pyrococcus horikoshii
1.1.1.87
DNA and amino acid sequence determination and analysis, expression of His-tagged enzyme in Escherichia coli
Pyrococcus horikoshii
1.1.1.286
Pyrococcus horikoshii
KM Value [mM]
1.1.1.41
0.0164
isocitrate
6fold His-tagged enzyme, at 70°C in 50 mM N-2-hydroxyethylpiperadine-N0-2-ethanesulfonate-NaOH (pH 7.8), 0.2 M KCl, 0.2 mM MnCl2, 1mM NAD+
Pyrococcus horikoshii
1.1.1.41
0.0183
homoisocitrate
6fold His-tagged enzyme, at 70°C in 50 mM N-2-hydroxyethylpiperadine-N0-2-ethanesulfonate-NaOH (pH 7.8), 0.2 M KCl, 0.2 mM MnCl2, 1mM NAD+
Pyrococcus horikoshii
1.1.1.41
0.0771
NAD+
6fold His-tagged enzyme, at 70°C in 50 mM N-2-hydroxyethylpiperadine-N0-2-ethanesulfonate-NaOH (pH 7.8), 0.2 M KCl, 0.2 mM MnCl2
Pyrococcus horikoshii
1.1.1.87
0.0164
isocitrate
pH 7.8, 70°C, recombinant enzyme
Pyrococcus horikoshii
1.1.1.87
0.0183
homoisocitrate
pH 7.8, 70°C, recombinant enzyme
Pyrococcus horikoshii
1.1.1.286
0.0164
isocitrate
70°C, pH 7.8
Pyrococcus horikoshii
1.1.1.286
0.0183
homoisocitrate
70°C, pH 7.8
Pyrococcus horikoshii
1.1.1.286
0.0771
NAD+
70°C, pH 7.8
Pyrococcus horikoshii
Metals/Ions
1.1.1.41
K+
Pyrococcus horikoshii
1.1.1.41
Mn2+
Pyrococcus horikoshii
1.1.1.87
Mn2+
Pyrococcus horikoshii
Natural Substrates/ Products (Substrates)
1.1.1.87
3-isopropylmalate + NAD+
Pyrococcus horikoshii
?
?
1.1.1.87
homoisocitrate + NAD+
Pyrococcus horikoshii
?
?
1.1.1.87
isocitrate + NAD+
Pyrococcus horikoshii
?
?
1.1.1.87
additional information
Pyrococcus horikoshii
the enzyme is trifunctional performing the activities of 3-isopropylmalate, isocitrate, and homoisocitrate dehydrogenase in one pathway
?
?
Organism
1.1.1.41
Pyrococcus horikoshii
1.1.1.87
Pyrococcus horikoshii
A4CYJ9
gene PH1722 or hdh
1.1.1.286
Pyrococcus horikoshii
expression in Escherichia coli
Purification (Commentary)
1.1.1.41
Hi-Trap chelating HP column chromatography
Pyrococcus horikoshii
1.1.1.87
recombinnat His-tagged enzyme from Escherichia coli by metal affinity chromatography
Pyrococcus horikoshii
1.1.1.286
recombinant enzyme expressed in Escherichia coli, purification of inclusion bodies requires N-laurylsarcosine
Pyrococcus horikoshii
Renatured (Commentary)
1.1.1.41
using N-lauroylsarcosine to 0.3% v/v
Pyrococcus horikoshii
Specific Activity [micromol/min/mg]
1.1.1.41
additional information
no activity on 3-isopropylmalate
Pyrococcus horikoshii
1.1.1.87
additional information
Pyrococcus horikoshii
Substrates and Products (Substrate)
1.1.1.41
D-homoisocitrate + NAD+
660907
Pyrococcus horikoshii
? + NADH
?
1.1.1.41
D-isocitrate + NAD+
660907
Pyrococcus horikoshii
2-oxoglutarate + CO2 + NADH
?
1.1.1.41
isocitrate + NAD+
660907
Pyrococcus horikoshii
2-oxoglutarate + CO2 + NADH
r
1.1.1.87
3-isopropylmalate + NAD+
660907
Pyrococcus horikoshii
?
?
1.1.1.87
homoisocitrate + NAD+
660907
Pyrococcus horikoshii
?
?
1.1.1.87
isocitrate + NAD+
660907
Pyrococcus horikoshii
?
?
1.1.1.87
additional information
the enzyme is trifunctional performing the activities of 3-isopropylmalate, isocitrate, and homoisocitrate dehydrogenase in one pathway
660907
Pyrococcus horikoshii
?
?
1.1.1.286
homoisocitrate + NAD+
660907
Pyrococcus horikoshii
? + NADH
?
1.1.1.286
isocitrate + NAD+
about 20times more efficient than homoisocitrate, NAD+ is preferred over NADP+
660907
Pyrococcus horikoshii
2-oxoglutarate + CO2 + NADH
?
1.1.1.286
isocitrate + NADP+
NAD+ is preferred over NADP+
660907
Pyrococcus horikoshii
2-oxoglutarate + CO2 + NADPH
?
1.1.1.286
additional information
no substrate: 3-isopropylmalate
660907
Pyrococcus horikoshii
?
?
Synonyms
1.1.1.41
beta-decarboxylating dehydrogenase
Pyrococcus horikoshii
1.1.1.41
isocitrate-homoisocitrate dehydrogenase
Pyrococcus horikoshii
1.1.1.41
PH1722
Pyrococcus horikoshii
1.1.1.87
HICDH
Pyrococcus horikoshii
1.1.1.87
isocitrate-homoisocitrate dehydrogenase
Pyrococcus horikoshii
1.1.1.87
protein PH1722
Pyrococcus horikoshii
Temperature Optimum [°C]
1.1.1.87
70
assay at
Pyrococcus horikoshii
Turnover Number [1/s]
1.1.1.41
13.7
homoisocitrate
6fold His-tagged enzyme, at 70°C in 50 mM N-2-hydroxyethylpiperadine-N0-2-ethanesulfonate-NaOH (pH 7.8), 0.2 M KCl, 0.2 mM MnCl2, 1 mM NAD+
Pyrococcus horikoshii
1.1.1.41
14.8
isocitrate
6fold His-tagged enzyme, at 70°C in 50 mM N-2-hydroxyethylpiperadine-N0-2-ethanesulfonate-NaOH (pH 7.8), 0.2 M KCl, 0.2 mM MnCl2, 1 mM NAD+
Pyrococcus horikoshii
1.1.1.41
21.8
NAD+
6fold His-tagged enzyme, at 70°C in 50 mM N-2-hydroxyethylpiperadine-N0-2-ethanesulfonate-NaOH (pH 7.8), 0.2 M KCl, 0.2 mM MnCl2
Pyrococcus horikoshii
1.1.1.87
13.7
homoisocitrate
pH 7.8, 70°C, recombinant enzyme
Pyrococcus horikoshii
1.1.1.87
14.8
isocitrate
pH 7.8, 70°C, recombinant enzyme
Pyrococcus horikoshii
1.1.1.286
13.7
homoisocitrate
70°C, pH 7.8
Pyrococcus horikoshii
1.1.1.286
14.8
isocitrate
70°C, pH 7.8
Pyrococcus horikoshii
1.1.1.286
21.8
NAD+
70°C, pH 7.8
Pyrococcus horikoshii
pH Optimum
1.1.1.87
7.8
assay at
Pyrococcus horikoshii
Cofactor
1.1.1.41
NAD+
1 mM
Pyrococcus horikoshii
1.1.1.87
NAD+
Pyrococcus horikoshii
Cloned(Commentary) (protein specific)
1.1.1.41
expressed in Escherichia coli strain BL21-CodonPlus (DE3)-RIL
Pyrococcus horikoshii
1.1.1.87
DNA and amino acid sequence determination and analysis, expression of His-tagged enzyme in Escherichia coli
Pyrococcus horikoshii
1.1.1.286
Pyrococcus horikoshii
Cofactor (protein specific)
1.1.1.41
NAD+
1 mM
Pyrococcus horikoshii
1.1.1.87
NAD+
Pyrococcus horikoshii
KM Value [mM] (protein specific)
1.1.1.41
0.0164
isocitrate
6fold His-tagged enzyme, at 70°C in 50 mM N-2-hydroxyethylpiperadine-N0-2-ethanesulfonate-NaOH (pH 7.8), 0.2 M KCl, 0.2 mM MnCl2, 1mM NAD+
Pyrococcus horikoshii
1.1.1.41
0.0183
homoisocitrate
6fold His-tagged enzyme, at 70°C in 50 mM N-2-hydroxyethylpiperadine-N0-2-ethanesulfonate-NaOH (pH 7.8), 0.2 M KCl, 0.2 mM MnCl2, 1mM NAD+
Pyrococcus horikoshii
1.1.1.41
0.0771
NAD+
6fold His-tagged enzyme, at 70°C in 50 mM N-2-hydroxyethylpiperadine-N0-2-ethanesulfonate-NaOH (pH 7.8), 0.2 M KCl, 0.2 mM MnCl2
Pyrococcus horikoshii
1.1.1.87
0.0164
isocitrate
pH 7.8, 70°C, recombinant enzyme
Pyrococcus horikoshii
1.1.1.87
0.0183
homoisocitrate
pH 7.8, 70°C, recombinant enzyme
Pyrococcus horikoshii
1.1.1.286
0.0164
isocitrate
70°C, pH 7.8
Pyrococcus horikoshii
1.1.1.286
0.0183
homoisocitrate
70°C, pH 7.8
Pyrococcus horikoshii
1.1.1.286
0.0771
NAD+
70°C, pH 7.8
Pyrococcus horikoshii
Metals/Ions (protein specific)
1.1.1.41
K+
Pyrococcus horikoshii
1.1.1.41
Mn2+
Pyrococcus horikoshii
1.1.1.87
Mn2+
Pyrococcus horikoshii
Natural Substrates/ Products (Substrates) (protein specific)
1.1.1.87
3-isopropylmalate + NAD+
Pyrococcus horikoshii
?
?
1.1.1.87
homoisocitrate + NAD+
Pyrococcus horikoshii
?
?
1.1.1.87
isocitrate + NAD+
Pyrococcus horikoshii
?
?
1.1.1.87
additional information
Pyrococcus horikoshii
the enzyme is trifunctional performing the activities of 3-isopropylmalate, isocitrate, and homoisocitrate dehydrogenase in one pathway
?
?
Purification (Commentary) (protein specific)
1.1.1.41
Hi-Trap chelating HP column chromatography
Pyrococcus horikoshii
1.1.1.87
recombinnat His-tagged enzyme from Escherichia coli by metal affinity chromatography
Pyrococcus horikoshii
1.1.1.286
recombinant enzyme expressed in Escherichia coli, purification of inclusion bodies requires N-laurylsarcosine
Pyrococcus horikoshii
Renatured (Commentary) (protein specific)
1.1.1.41
using N-lauroylsarcosine to 0.3% v/v
Pyrococcus horikoshii
Specific Activity [micromol/min/mg] (protein specific)
1.1.1.41
additional information
no activity on 3-isopropylmalate
Pyrococcus horikoshii
1.1.1.87
additional information
Pyrococcus horikoshii
Substrates and Products (Substrate) (protein specific)
1.1.1.41
D-homoisocitrate + NAD+
660907
Pyrococcus horikoshii
? + NADH
?
1.1.1.41
D-isocitrate + NAD+
660907
Pyrococcus horikoshii
2-oxoglutarate + CO2 + NADH
?
1.1.1.41
isocitrate + NAD+
660907
Pyrococcus horikoshii
2-oxoglutarate + CO2 + NADH
r
1.1.1.87
3-isopropylmalate + NAD+
660907
Pyrococcus horikoshii
?
?
1.1.1.87
homoisocitrate + NAD+
660907
Pyrococcus horikoshii
?
?
1.1.1.87
isocitrate + NAD+
660907
Pyrococcus horikoshii
?
?
1.1.1.87
additional information
the enzyme is trifunctional performing the activities of 3-isopropylmalate, isocitrate, and homoisocitrate dehydrogenase in one pathway
660907
Pyrococcus horikoshii
?
?
1.1.1.286
homoisocitrate + NAD+
660907
Pyrococcus horikoshii
? + NADH
?
1.1.1.286
isocitrate + NAD+
about 20times more efficient than homoisocitrate, NAD+ is preferred over NADP+
660907
Pyrococcus horikoshii
2-oxoglutarate + CO2 + NADH
?
1.1.1.286
isocitrate + NADP+
NAD+ is preferred over NADP+
660907
Pyrococcus horikoshii
2-oxoglutarate + CO2 + NADPH
?
1.1.1.286
additional information
no substrate: 3-isopropylmalate
660907
Pyrococcus horikoshii
?
?
Temperature Optimum [°C] (protein specific)
1.1.1.87
70
assay at
Pyrococcus horikoshii
Turnover Number [1/s] (protein specific)
1.1.1.41
13.7
homoisocitrate
6fold His-tagged enzyme, at 70°C in 50 mM N-2-hydroxyethylpiperadine-N0-2-ethanesulfonate-NaOH (pH 7.8), 0.2 M KCl, 0.2 mM MnCl2, 1 mM NAD+
Pyrococcus horikoshii
1.1.1.41
14.8
isocitrate
6fold His-tagged enzyme, at 70°C in 50 mM N-2-hydroxyethylpiperadine-N0-2-ethanesulfonate-NaOH (pH 7.8), 0.2 M KCl, 0.2 mM MnCl2, 1 mM NAD+
Pyrococcus horikoshii
1.1.1.41
21.8
NAD+
6fold His-tagged enzyme, at 70°C in 50 mM N-2-hydroxyethylpiperadine-N0-2-ethanesulfonate-NaOH (pH 7.8), 0.2 M KCl, 0.2 mM MnCl2
Pyrococcus horikoshii
1.1.1.87
13.7
homoisocitrate
pH 7.8, 70°C, recombinant enzyme
Pyrococcus horikoshii
1.1.1.87
14.8
isocitrate
pH 7.8, 70°C, recombinant enzyme
Pyrococcus horikoshii
1.1.1.286
13.7
homoisocitrate
70°C, pH 7.8
Pyrococcus horikoshii
1.1.1.286
14.8
isocitrate
70°C, pH 7.8
Pyrococcus horikoshii
1.1.1.286
21.8
NAD+
70°C, pH 7.8
Pyrococcus horikoshii
pH Optimum (protein specific)
1.1.1.87
7.8
assay at
Pyrococcus horikoshii