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Literature summary extracted from

  • Marina, P.; Martinez-Costa, O.H.; Calderon, I.L.; Aragon, J.J.
    Characterization of the aspartate kinase from Saccharomyces cerevisiae and of its interaction with threonine (2004), Biochem. Biophys. Res. Commun., 321, 584-591.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.2.4 expression in Escherichia coli and as fusion protein with GST (glutathione-S-transferase) in Saccharomyces cerevisiae Saccharomyces cerevisiae

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.2.4 L-threonine allosteric inhibition, causes a decrease in the apparent molecular mass of the enzyme, no inhibition is detected in the threonine insensitive mutant up to 50 mM threonine Saccharomyces cerevisiae

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.2.4 0.8
-
 ATP wild type, in the presence of 10 mM aspartate Saccharomyces cerevisiae
2.7.2.4 1.1
-
 L-aspartate threonine insensitive mutant, in the presence of 10 mM ATP Saccharomyces cerevisiae
2.7.2.4 1.1
-
 L-aspartate wildtype, in the presence of 10 mM ATP Saccharomyces cerevisiae
2.7.2.4 1.2
-
 ATP glutathione-S-transferase fusion protein, in the presence of 10 mM aspartate Saccharomyces cerevisiae
2.7.2.4 1.2
-
 L-aspartate glutathione-S-transferase fusion protein, in the presence of 10 mM ATP Saccharomyces cerevisiae
2.7.2.4 1.2
-
 ATP threonine insensitive mutant, in the presence of 10 mM aspartate Saccharomyces cerevisiae

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.2.4 58000
-
 wild type, gel filtration Saccharomyces cerevisiae
2.7.2.4 58100
-
 wild type, calculated from protein sequence Saccharomyces cerevisiae
2.7.2.4 58700
-
 wild type, SDS-PAGE Saccharomyces cerevisiae
2.7.2.4 280000
-
 glutathione-S-transferase fusion protein, in the presence of threonine, gel filtration Saccharomyces cerevisiae
2.7.2.4 297000
-
 glutathione-S-transferase fusion protein, in the presence of threonine, blue native gel electrophoresis Saccharomyces cerevisiae
2.7.2.4 298000
-
 wild type, in the presence of threonine, gel filtration Saccharomyces cerevisiae
2.7.2.4 344000
-
 glutathione-S-transferase fusion protein, gel filtration Saccharomyces cerevisiae
2.7.2.4 344000
-
 threonine insensitive mutant, with and without threonine in the buffer, blue native gel electrophoresis Saccharomyces cerevisiae
2.7.2.4 345000
-
 threonine insensitive mutant, with and without threonine in the buffer, gel filtration Saccharomyces cerevisiae
2.7.2.4 345000
-
 wild type, blue native gel electrophoresis, gel filtration Saccharomyces cerevisiae
2.7.2.4 346000
-
 wild type, gel filtration Saccharomyces cerevisiae
2.7.2.4 346000
-
 glutathione-S-transferase fusion protein, blue native gel electrophoresis Saccharomyces cerevisiae

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.2.4 ATP + L-aspartate Saccharomyces cerevisiae
-
 ADP + phospho-L-aspartate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.7.2.4 Saccharomyces cerevisiae
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.2.4
-
 Saccharomyces cerevisiae

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.7.2.4 39.05
-
 activity after the final purification step Saccharomyces cerevisiae

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.2.4 ATP + L-aspartate
-
 Saccharomyces cerevisiae ADP + phospho-L-aspartate
-
 ?

Subunits

EC Number Subunits Comment Organism
2.7.2.4 hexamer 6 * 58000, gel filtration, native gel electrophoresis Saccharomyces cerevisiae
2.7.2.4 pentamer glutathione-S-transferase fusion protein, in the presence of threonine, gel filtration Saccharomyces cerevisiae

Synonyms

EC Number Synonyms Comment Organism
2.7.2.4 AK
-
 Saccharomyces cerevisiae
2.7.2.4 aspartate kinase
-
 Saccharomyces cerevisiae

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.7.2.4 227
-
 L-aspartate
-
 Saccharomyces cerevisiae

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
2.7.2.4 1.4
-
 L-threonine glutathione-S-transferase fusion protein, in the presence of 10 mM aspartate and 10 mM ATP Saccharomyces cerevisiae
2.7.2.4 1.4
-
 L-threonine wildtype, in the presence of 10 mM aspartate and 10 mM ATP, binds threonine in a hyperbolic manner Saccharomyces cerevisiae