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Literature summary extracted from

  • Fujii, K.; Minagawa, H.; Terada, Y.; Takaha, T.; Kuriki, T.; Shimada, J.; Kaneko, H.
    Use of random and saturation mutageneses to improve the properties of Thermus aquaticus amylomaltase for efficient production of cycloamyloses (2005), Appl. Environ. Microbiol., 71, 5823-5827.
    View publication on PubMedView publication on EuropePMC

Application

EC Number Application Comment Organism
2.4.1.25 biotechnology industrial production of cycloamylase with mutant enzyme Y54G, which shows high cyclization activity and low hydrolytic activity Thermus aquaticus

Protein Variants

EC Number Protein Variants Comment Organism
2.4.1.25 Y54A hydrolytic activity is 39% of the wild-type value, cyclization activity is 192% of the wild-type value Thermus aquaticus
2.4.1.25 Y54C hydrolytic activity is 48% of the wild-type value, cyclization activity is 122% of the wild-type value Thermus aquaticus
2.4.1.25 Y54D hydrolytic activity is 38% of the wild-type value, cyclization activity is 177% of the wild-type value Thermus aquaticus
2.4.1.25 Y54E hydrolytic activity is 48% of the wild-type value, cyclization activity is 157% of the wild-type value Thermus aquaticus
2.4.1.25 Y54F hydrolytic activity is 96% of the wild-type value, cyclization activity is 91% of the wild-type value Thermus aquaticus
2.4.1.25 Y54G hydrolytic activity is 16% of the wild-type value, cyclization activity is 167% of the wild-type value Thermus aquaticus
2.4.1.25 Y54H hydrolytic activity is 76% of the wild-type value, cyclization activity is 164% of the wild-type value Thermus aquaticus
2.4.1.25 Y54I hydrolytic activity is 33% of the wild-type value, cyclization activity is 157% of the wild-type value Thermus aquaticus
2.4.1.25 Y54K hydrolytic activity is 99% of the wild-type value, cyclization activity is 176% of the wild-type value Thermus aquaticus
2.4.1.25 Y54L hydrolytic activity is 39% of the wild-type value, cyclization activity is 189% of the wild-type value Thermus aquaticus
2.4.1.25 Y54M hydrolytic activity is 39% of the wild-type value, cyclization activity is 81% of the wild-type value Thermus aquaticus
2.4.1.25 Y54N hydrolytic activity is 30% of the wild-type value, cyclization activity is 159% of the wild-type value Thermus aquaticus
2.4.1.25 Y54P hydrolytic activity is 5% of the wild-type value, cyclization activity is 48% of the wild-type value Thermus aquaticus
2.4.1.25 Y54Q hydrolytic activity is 70% of the wild-type value, cyclization activity is 168% of the wild-type value Thermus aquaticus
2.4.1.25 Y54R hydrolytic activity is 70% of the wild-type value, cyclization activity is 200% of the wild-type value Thermus aquaticus
2.4.1.25 Y54S hydrolytic activity is 38% of the wild-type value, cyclization activity is 186% of the wild-type value Thermus aquaticus
2.4.1.25 Y54T hydrolytic activity is 22% of the wild-type value, cyclization activity is 149% of the wild-type value Thermus aquaticus
2.4.1.25 Y54V hydrolytic activity is 29% of the wild-type value, cyclization activity is 160% of the wild-type value Thermus aquaticus
2.4.1.25 Y54W hydrolytic activity is 20% of the wild-type value, cyclization activity is 95% of the wild-type value Thermus aquaticus

Organism

EC Number Organism UniProt Comment Textmining
2.4.1.25 Thermus aquaticus
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.1.25 amylose + ?
-
 Thermus aquaticus cycloamylose + ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.4.1.25 amylomaltase
-
 Thermus aquaticus