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Literature summary extracted from

  • Roberts, J.L.; Moretti, P.A.B.; Darrow, A.L.; Derian, C.K.; Vadas, M.A.; Pitson, S.M.
    An assay for sphingosine kinase activity using biotinylated sphingosine and streptavidin-coated membranes (2004), Anal. Biochem., 331, 122-129.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.7.1.91 additional information solubilization in Triton X-100 results in higher activity compared to bovine serum albumin Homo sapiens
2.7.1.91 phorbol 12-myristate 13-acetate
-
 Homo sapiens
2.7.1.91 TNF-alpha
-
 Homo sapiens

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.1.91 expression of FLAG-tagged isozyme in HEK293 cells Homo sapiens
2.7.1.91 expression of FLAG-tagged isozyme in HEK293 cells, expression of His-tagged isozyme in Sf9 insect cells via baculovirus infection system Homo sapiens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.1.91 additional information
-
 additional information substrate kinetic analysis Homo sapiens

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.1.91 Mg2+
-
 Homo sapiens

Organism

EC Number Organism UniProt Comment Textmining
2.7.1.91 Homo sapiens Q9NRA0 isozyme Sk2; 2 isozymes SK1 and SK2
-
2.7.1.91 Homo sapiens Q9NYA1 isozyme Sk1; 2 isozymes SK1 and SK2
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.7.1.91 0.184
-
 native isozyme SK1 in cell extract Homo sapiens
2.7.1.91 19.34
-
 purified recombinant isozyme SK1 Homo sapiens
2.7.1.91 43.4
-
 recombinant isozyme SK1 in cell extract of recombinant cells Homo sapiens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.1.91 ATP + biotinyl-D-erythro-sphingosine
-
 Homo sapiens ADP + biotinyl-D-erythro-sphingosine 1-phosphate
-
 ?
2.7.1.91 ATP + D-erythro-sphingosine
-
 Homo sapiens ADP + D-erythro-sphingosine 1-phosphate
-
 ?
2.7.1.91 additional information isozyme SK1 shows high activity compared to isozyme SK2, development and optimization of a high-sensitive assay method utilizing biotinyl sphingosine and streptavidin-coated membranes with recombinant isozymes Homo sapiens ?
-
 ?
2.7.1.91 additional information isozyme SK2 shows low activity compared to isozyme SK1, development and optimization of a high-sensitive assay method utilizing biotinyl-sphingosine and streptavidin-coated membranes with recombinant isozymes Homo sapiens ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.7.1.91 SK
-
 Homo sapiens
2.7.1.91 sphinganine kinase
-
 Homo sapiens

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.7.1.91 37
-
 assay at Homo sapiens

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.1.91 7.4
-
 assay at Homo sapiens

Cofactor

EC Number Cofactor Comment Organism Structure
2.7.1.91 ATP
-
 Homo sapiens