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Literature summary extracted from

  • Joo, G.J.; Shin, J.H.; Heo, G.Y.; Kwak, Y.Y.; Choi, J.H.; Rhee, I.K.
    Purification and characterization of a thermostable xylose (glucose) isomerase from Streptomyces chibaensis J-59 (2001), Agric. Chem. Biotechnol., 44, 113-118.
No PubMed abstract available

Inhibitors

EC Number Inhibitors Comment Organism Structure
5.3.1.5 Ag+
-
 Streptomyces corchorusii
5.3.1.5 Ca2+ no inhibitory effect Streptomyces corchorusii
5.3.1.5 Cu2+
-
 Streptomyces corchorusii
5.3.1.5 Hg2+
-
 Streptomyces corchorusii

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
5.3.1.5 83
-
 D-glucose
-
 Streptomyces corchorusii

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
5.3.1.5 Co2+ required for activity Streptomyces corchorusii
5.3.1.5 Mg2+ required for activity, most effective cation Streptomyces corchorusii
5.3.1.5 Mn2+ required for activity Streptomyces corchorusii

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
5.3.1.5 44000
-
 SDS-PAGE, monomer Streptomyces corchorusii
5.3.1.5 180000
-
 gel filtration Streptomyces corchorusii

Organism

EC Number Organism UniProt Comment Textmining
5.3.1.5 Streptomyces corchorusii
-
-
-
5.3.1.5 Streptomyces corchorusii J-59
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
5.3.1.5 to homogeneity Streptomyces corchorusii

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
5.3.1.5 40.9
-
-
 Streptomyces corchorusii

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5.3.1.5 D-Glucose
-
 Streptomyces corchorusii D-Fructose
-
 ?
5.3.1.5 D-Glucose
-
 Streptomyces corchorusii J-59 D-Fructose
-
 ?

Subunits

EC Number Subunits Comment Organism
5.3.1.5 tetramer alpha4, 4 * 44000, SDS-PAGE, gel filtration Streptomyces corchorusii

Synonyms

EC Number Synonyms Comment Organism
5.3.1.5 xylose (glucose) isomerase
-
 Streptomyces corchorusii

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
5.3.1.5 85
-
-
 Streptomyces corchorusii

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
5.3.1.5 70
-
 in the presence of CO2+, cell-free xylose isomerase retains 100% activity without loss of activity for 7 days Streptomyces corchorusii
5.3.1.5 85
-
 in the presence of CO2+, cell-free xylose isomerase retains 50% residual activity for 13.5 h Streptomyces corchorusii
5.3.1.5 90
-
 in the presence of CO2+, cell-free xylose isomerase retains 50% residual activity for 126 min Streptomyces corchorusii

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
5.3.1.5 30.72
-
 D-glucose
-
 Streptomyces corchorusii

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
5.3.1.5 7.5
-
-
 Streptomyces corchorusii