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Literature summary extracted from

  • Jeong, J.J.; Fushinobu, S.; Ito, S.; Shoun, H.; Wakagi, T.
    Archaeal ADP-dependent phosphofructokinase: expression, purification, crystallization and preliminary crystallographic analysis (2003), Acta Crystallogr. Sect. D, 59, 1327-1329.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.1.146 DNA and amino acid sequence determination and analysis, expression in Escherichia coli strain JM109 Thermococcus litoralis

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
2.7.1.146 purified recombinant enzyme, sitting drop vapour diffusion method, 0.005 ml of 10 mg/ml protein in 150 mM NaCl, 5 mM Tris-HCl, pH 7.5, is mixed with an equal volume of reservoir solution containing 4 M NaCl, 100 mM Tris-HCl, pH 7.5, 1 week at 25°C, derivatization by soaking for 2 days in 2.85 mM K2PtCl4, 2.8 mM HAuCl4, and 2 mM 4-hydroxymercuribenzoic acid or 0.4 mM 4-hydroxymercuribenzenesulfonic acid, cryoprotection by 20% glycerol in mother liquor, X-ray diffraction structure determination and analysis at 2.6 A resolution Thermococcus litoralis

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.1.146 2.56
-
 D-fructose 6-phosphate
-
 Thermococcus litoralis

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.1.146 Mg2+ required Thermococcus litoralis

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.1.146 52000
-
 2 * 52000, recombinant enzyme, SDS-PAGE Thermococcus litoralis
2.7.1.146 125000
-
 recombinant enzyme, gel filtration Thermococcus litoralis

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.1.146 ADP + D-fructose 6-phosphate Thermococcus litoralis
-
 AMP + D-fructose 1,6-bisphosphate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.7.1.146 Thermococcus litoralis Q977Q3
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.1.146 recombinant enzyme from Escherichia coli strain JM109 by ammonium sulfate fractionation, adsorption and ion exchange chromatography, and gel filtration Thermococcus litoralis

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.7.1.146 157
-
 purified recombinant enzyme Thermococcus litoralis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.1.146 ADP + D-fructose 6-phosphate
-
 Thermococcus litoralis AMP + D-fructose 1,6-bisphosphate
-
 ?

Subunits

EC Number Subunits Comment Organism
2.7.1.146 dimer 2 * 52000, recombinant enzyme, SDS-PAGE Thermococcus litoralis

Synonyms

EC Number Synonyms Comment Organism
2.7.1.146 ADP-dependent phosphofructokinase
-
 Thermococcus litoralis
2.7.1.146 TLPFK
-
 Thermococcus litoralis

Cofactor

EC Number Cofactor Comment Organism Structure
2.7.1.146 ADP
-
 Thermococcus litoralis
2.7.1.146 additional information no activity with ATP Thermococcus litoralis