Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Furnes, B.; Schlenk, D.
    Evaluation of xenobiotic N- and S-oxidation by variant flavin-containing monooxygenase 1 (FMO1) enzymes (2004), Toxicol. Sci., 78, 196-203.
    View publication on PubMed

Protein Variants

EC Number Protein Variants Comment Organism
1.14.13.8 D132H KM-value and Vmax of fenthion are nearly identical to the wild-type values, mutant of FMO3 Homo sapiens
1.14.13.8 H97Q KM-value for methimazole is 3fold higher than wild-type value, Vmax with methimazole is 1.6fold higher than the wild-type value, KM-value for methyl p-tolyl sulfide is 88% of the wild-type value, Vmax with methyl p-tolyl sulfide is 1.4fold higher than the wild-type value, KM-value for imipramine is is nearly identical to the the wild-type value, Vmax with imipramine is 1.3fold higher than the wild-type value, KM-value for fenthion is 94% of the wild-type value, Vmax with fenthion is 1.3fold higher than the wild-type value, mutant of FMO1 Homo sapiens
1.14.13.8 I303T KM-value for methimazole is 2.3fold higher than wild-type value, Vmax with methimazole is 1.8fold higher than the wild-type value, KM-value for methyl p-tolyl sulfide is 86% of the wild-type value, Vmax with methyl p-tolyl sulfide is 1.8fold higher than the wild-type value, KM-value for imipramine is identical to the the wild-type value, Vmax with imipramine is 1.4fold higher than the wild-type value, KM-value for fenthion is 94% of the wild-type value, Vmax with fenthion is 1.6fold higher than the wild-type value, mutant of FMO1 Homo sapiens
1.14.13.8 I303V KM-value for methimazole is identical to the wild-type value, Vmax with methimazole is nearly identical to the wild-type value, KM-value and Vmax for meth is 1,4fold higher than the the wild-type value, Vmax with imipramine is nearly identical to the wild-type value, KM-value and Vmax for fenthion are nearly identical to the wild-type values, mutant of FMO1 Homo sapiens
1.14.13.8 K158L Km-value for fenthion is 1.4fold higher than the wild-type value, Vmax for fenthion is nearly identical to the wild-type value, mutant of FMO3 Homo sapiens
1.14.13.8 K158L/D132H Km-value for fenthion is 1.5fold higher than the wild-type value, Vmax for fenthion is 1.5fold higher than the wild-type value, mutant of FMO3 Homo sapiens
1.14.13.8 R502V no activity with methimazole, KM-value for methyl p-tolyl sulfide is 70% of the wild-type value, Vmax with methyl p-tolyl sulfide is 70% of the wild-type value, KM-value for imipramine is is nearly identical to the the wild-type value, Vmax with imipramine is 49% of the wild-type value, KM-value for fenthion is 88% of the wild-type value, Vmax with fenthion is 55% of wild-type value, mutant of FMO1 Homo sapiens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.14.13.8 0.007
-
 Methimazole mutant enzyme I303V, FMO1 Homo sapiens
1.14.13.8 0.007
-
 Methimazole wild-type enzyme, FMO1 Homo sapiens
1.14.13.8 0.014
-
 Methimazole mutant enzyme H97Q, FMO1 Homo sapiens
1.14.13.8 0.014
-
 imipramine mutant enzyme I303T, FMO1 Homo sapiens
1.14.13.8 0.014
-
 imipramine wild-type enzyme, FMO1 Homo sapiens
1.14.13.8 0.015
-
 imipramine mutant enzyme H97Q, FMO1 Homo sapiens
1.14.13.8 0.016
-
 Methimazole mutant enzyme I303T, FMO1 Homo sapiens
1.14.13.8 0.016
-
 imipramine mutant enzyme R502X, FMO1 Homo sapiens
1.14.13.8 0.02
-
 imipramine mutant enzyme I303V, FMO1 Homo sapiens
1.14.13.8 0.145
-
 fenthion wild-type enzyme, FMO3 Homo sapiens
1.14.13.8 0.15
-
 fenthion mutant enzyme D132H, FMO3 Homo sapiens
1.14.13.8 0.2
-
 fenthion mutant enzyme K158L, FMO3 Homo sapiens
1.14.13.8 0.2
-
 methyl p-tolyl sulfide mutant enzyme R502X, FMO1 Homo sapiens
1.14.13.8 0.22
-
 fenthion mutant enzyme K158L/D132H, FMO3 Homo sapiens
1.14.13.8 0.24
-
 fenthion mutant enzyme I303T, FMO1 Homo sapiens
1.14.13.8 0.245
-
 methyl p-tolyl sulfide mutant enzyme I303T, FMO1 Homo sapiens
1.14.13.8 0.251
-
 methyl p-tolyl sulfide mutant enzyme H97Q, FMO1 Homo sapiens
1.14.13.8 0.284
-
 methyl p-tolyl sulfide wild-type enzyme, FMO1 Homo sapiens
1.14.13.8 0.3
-
 methyl p-tolyl sulfide mutant enzyme I303V, FMO1 Homo sapiens
1.14.13.8 0.3
-
 fenthion mutant enzyme R502X, FMO1 Homo sapiens
1.14.13.8 0.32
-
 fenthion mutant enzyme H97Q, FMO1 Homo sapiens
1.14.13.8 0.34
-
 fenthion wild-type enzyme, FMO1 Homo sapiens
1.14.13.8 0.351
-
 fenthion mutant enzyme I303V, FMO1 Homo sapiens

Organism

EC Number Organism UniProt Comment Textmining
1.14.13.8 Homo sapiens
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.13.8 fenthion + NADPH + O2
-
 Homo sapiens fenthion sulfoxide + NADP+ 74% (+)-sulfoxide ?
1.14.13.8 fenthion + NADPH + O2
-
 Homo sapiens fenthion sulfoxide + NADP+ + H2O more than 95% (+)-sulfoxide ?
1.14.13.8 imipramine + NADPH + O2
-
 Homo sapiens imipramine N-oxide + NADP+ + H2O
-
 ?
1.14.13.8 methimazole + NADPH + O2
-
 Homo sapiens N-methylmethimidazole-2-sulfinic acid + NADP+ + H2O
-
 ?
1.14.13.8 methyl p-tolyl sulfide + NADPH + O2
-
 Homo sapiens methyl p-tolyl sulfoxide + NADP+ + H2O
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.14.13.8 flavin-containing monooxygenase 1
-
 Homo sapiens
1.14.13.8 flavin-containing monooxygenase 3
-
 Homo sapiens
1.14.13.8 FMO1
-
 Homo sapiens
1.14.13.8 FMO3
-
 Homo sapiens

Cofactor

EC Number Cofactor Comment Organism Structure
1.14.13.8 NADPH
-
 Homo sapiens