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Literature summary extracted from

  • Kakuda, S.; Oka, S.; Kawasaki, T.
    Purification and characterization of two recombinant human glucuronyltransferases involved in the biosynthesis of HNK-1 carbohydrate in Escherichia coli (2004), Protein Expr. Purif., 35, 111-119.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.4.1.17 phosphatidylinositol highly required for activity of GlcAT-P and GlcAT-S with paraglobosid and other glycolipids Homo sapiens
2.4.1.17 sphingomyelin almost indispensable activator of GlcAT-P as to glycoprotein acceptors Homo sapiens

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.4.1.17 functional expression of FLAG-tagged enzymes GlcAT-P and GlcAT-S in Escherichia coli strain BL21 as soluble proteins Homo sapiens

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.4.1.17 asialo-orosomucoid inhibits activity with paragloboside Homo sapiens
2.4.1.17 lacto-N-biose 22% inhibition of recombinant GlcAT-P and 77.3% of recombinant GlcAT-S at 5 mM Homo sapiens
2.4.1.17 lactose 24.5% inhibition of recombinant GlcAT-S at 5 mM, no inhibition of recombinant GlcAT-P Homo sapiens
2.4.1.17 N-acetyllactosamine 87.4% inhibition of recombinant GlcAT-P and 83.7% of recombinant GlcAT-S at 5 mM Homo sapiens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.4.1.17 additional information
-
 additional information kinetics Homo sapiens

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.4.1.17 32000
-
 x * 32000, recombinant GlcAT-P and GlcAT-S, SDS-PAGE Homo sapiens

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.4.1.17 additional information Homo sapiens enzyme are involved in biosynthesis of HNK-1 carbohydrate ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.4.1.17 Homo sapiens
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.4.1.17 soluble recombinant FLAG-tagged enzymes GlcAT-P and GlcAT-S, 1233fold and 7511fold, respectively, from Escherichia coli by affinity chromatographic steps Homo sapiens

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.4.1.17 nervous system
-
 Homo sapiens
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.4.1.17 0.45
-
 purified recombinant GlcAT-S with substrate asialo-orosomucoid Homo sapiens
2.4.1.17 1.11
-
 purified recombinant GlcAT-P with substrate asialo-orosomucoid Homo sapiens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.1.17 additional information enzyme are involved in biosynthesis of HNK-1 carbohydrate Homo sapiens ?
-
 ?
2.4.1.17 additional information the recombinant GlcAT-P is highly specific for terminal type II structure, Galbeta1-4GlcNAc, while the recombinant GlcAT-S recognizes type I, Galbeta1-3GlcNAc, and type II structures Homo sapiens ?
-
 ?
2.4.1.17 UDP-glucuronate + asialo-orosomucoid acceptor is a glycoprotein, preferred substrate Homo sapiens UDP + asialo-orosomucoid beta-D-glucuronide
-
 ?
2.4.1.17 UDP-glucuronate + paragloboside acceptor is a glycolipid, activity requires phosphatidylinositol Homo sapiens UDP + paragloboside beta-D-glucuronide
-
 ?

Subunits

EC Number Subunits Comment Organism
2.4.1.17 ? x * 32000, recombinant GlcAT-P and GlcAT-S, SDS-PAGE Homo sapiens

Synonyms

EC Number Synonyms Comment Organism
2.4.1.17 GlcAT-P
-
 Homo sapiens
2.4.1.17 GlcAT-S
-
 Homo sapiens
2.4.1.17 glucuronyltransferase
-
 Homo sapiens

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.4.1.17 37
-
 assay at Homo sapiens

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.4.1.17 6.5
-
 assay at Homo sapiens