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Literature summary extracted from

  • Duclos, S.; Da Silva, P.; Vovelle, F.; Piller, F.; Piller, V.
    Characterization of the UDP-N-acetylgalactosamine binding domain of bovine polypeptide alphaN-acetylgalactosaminyltransferase T1 (2004), Protein Eng. Des. Sel., 17, 635-646.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.4.1.41 expression in Escherichia coli Bos taurus

Protein Variants

EC Number Protein Variants Comment Organism
2.4.1.41 D209A inactive mutant enzyme Bos taurus
2.4.1.41 F124A inactive mutant enzyme Bos taurus
2.4.1.41 L189A inactive mutant enzyme Bos taurus
2.4.1.41 N126A the turnover number for UDP-N-acetyl-D-galactosamine and STPSTPSTPSTPSTP is 1.4fold lower than the wild-type value, the KM-value for UDP-N-acetyl-D-galactosamine is 5.5fold higher than the wild-type value, the KM-value for STPSTPSTPSTPSTP is 1.6fold higher than the wild-type value Bos taurus
2.4.1.41 R193A inactive mutant enzyme Bos taurus
2.4.1.41 W129A the turnover number for UDP-N-acetyl-D-galactosamine and STPSTPSTPSTPSTP is 4.3fold lower than the wild-type value, the KM-value for UDP-N-acetyl-D-galactosamine is 3.5fold higher than the wild-type value, the KM-value for STPSTPSTPSTPSTP is 2.6fold higher than the wild-type value Bos taurus
2.4.1.41 W129F the turnover number for UDP-N-acetyl-D-galactosamine and STPSTPSTPSTPSTP is 1.4fold lower than the wild-type value, the KM-value for UDP-N-acetyl-D-galactosamine is 1.8fold higher than the wild-type value, the KM-value for STPSTPSTPSTPSTP is 1.2fold lower than the wild-type value Bos taurus
2.4.1.41 W129R the turnover number for UDP-N-acetyl-D-galactosamine and STPSTPSTPSTPSTP is 1.2fold lower than the wild-type value, the KM-value for UDP-N-acetyl-D-galactosamine is 2.1fold higher than the wild-type value, the KM-value for STPSTPSTPSTPSTP is 1.2fold lower than the wild-type value Bos taurus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.4.1.41 0.008
-
 UDP-N-acetyl-D-galactosamine pH 6.5, 37°C, wild-type enzyme Bos taurus
2.4.1.41 0.014
-
 UDP-N-acetyl-D-galactosamine pH 6.5, 37°C, mutant enzyme W129F Bos taurus
2.4.1.41 0.017
-
 UDP-N-acetyl-D-galactosamine pH 6.5, 37°C, mutant enzyme W129R Bos taurus
2.4.1.41 0.029
-
 UDP-N-acetyl-D-galactosamine pH 6.5, 37°C, mutant enzyme W129A Bos taurus
2.4.1.41 0.044
-
 UDP-N-acetyl-D-galactosamine pH 6.5, 37°C, mutant enzyme N126A Bos taurus
2.4.1.41 0.2
-
 STPSTPSTPSTPSTP pH 6.5, 37°C, mutant enzyme W129R Bos taurus
2.4.1.41 0.205
-
 STPSTPSTPSTPSTP pH 6.5, 37°C, mutant enzyme W129F Bos taurus
2.4.1.41 0.25
-
 STPSTPSTPSTPSTP pH 6.5, 37°C, wild-type enzyme Bos taurus
2.4.1.41 0.41
-
 STPSTPSTPSTPSTP pH 6.5, 37°C, mutant enzyme N126A Bos taurus
2.4.1.41 0.65
-
 STPSTPSTPSTPSTP pH 6.5, 37°C, mutant enzyme W129A Bos taurus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.4.1.41 Mn2+ in coordination both with the phosphates of UDP and the DXH motif of the enzyme Bos taurus

Organism

EC Number Organism UniProt Comment Textmining
2.4.1.41 Bos taurus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.4.1.41 recombinant enzyme Bos taurus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.1.41 UDP-N-acetyl-D-galactosamine + STPSTPSTPSTPSTP
-
 Bos taurus UDP + ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.4.1.41 ppGaNTase
-
 Bos taurus
2.4.1.41 UDP-GalNAc:polypeptide alphaN-acetylgalactosaminyltransferase
-
 Bos taurus

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.4.1.41 0.8
-
 UDP-N-acetyl-D-galactosamine pH 6.5, 37°C, mutant enzyme W129A Bos taurus
2.4.1.41 0.8
-
 STPSTPSTPSTPSTP pH 6.5, 37°C, mutant enzyme W129A Bos taurus
2.4.1.41 2.43
-
 UDP-N-acetyl-D-galactosamine pH 6.5, 37°C, mutant enzyme W129F Bos taurus
2.4.1.41 2.43
-
 STPSTPSTPSTPSTP pH 6.5, 37°C, mutant enzyme W129F Bos taurus
2.4.1.41 2.53
-
 UDP-N-acetyl-D-galactosamine pH 6.5, 37°C, mutant enzyme N126A Bos taurus
2.4.1.41 2.53
-
 STPSTPSTPSTPSTP pH 6.5, 37°C, mutant enzyme N126A Bos taurus
2.4.1.41 2.79
-
 UDP-N-acetyl-D-galactosamine pH 6.5, 37°C, mutant enzyme W129R Bos taurus
2.4.1.41 2.79
-
 STPSTPSTPSTPSTP pH 6.5, 37°C, mutant enzyme W129R Bos taurus
2.4.1.41 3.47
-
 UDP-N-acetyl-D-galactosamine pH 6.5, 37°C, wild-type enzyme Bos taurus
2.4.1.41 3.47
-
 STPSTPSTPSTPSTP pH 6.5, 37°C, wild-type enzyme Bos taurus