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Literature summary extracted from

  • Logan, D.T.; Mulliez, E.; Larsson, K.M.; Bodevin, S.; Atta, M.; Garnaud, P.E.; Sj๖berg, B.M.; Fontecave, M.
    A metal-binding site in the catalytic subunit of anaerobic ribonucleotide reductase (2003), Proc. Natl. Acad. Sci. USA, 100, 3826-3831.
    View publication on PubMedView publication on EuropePMC

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
1.1.98.6 C-terminal region contains a Zn(Cys)4 center structurally related to the zinc ribbon motif and to rubredoxin and rubrerythrin. Residues Cys543, Cys546, Cys561, and Cys564 coordinate the metal ion tetrahedrally Tequatrovirus T4
1.17.4.2
-
 Escherichia coli

Protein Variants

EC Number Protein Variants Comment Organism
1.1.98.6 C644A mutation in a residue of the Zn(Cys)4 center, almost complete loss of activity due to inability to generate the catalytically essential glycyl radical Escherichia coli
1.1.98.6 C647A mutation in a residue of the Zn(Cys)4 center, almost complete loss of activity due to inability to generate the catalytically essential glycyl radical Escherichia coli
1.1.98.6 C662A mutation in a residue of the Zn(Cys)4 center, almost complete loss of activity due to inability to generate the catalytically essential glycyl radical Escherichia coli
1.1.98.6 C665A mutation in a residue of the Zn(Cys)4 center, almost complete loss of activity due to inability to generate the catalytically essential glycyl radical Escherichia coli
1.17.4.2 C644A mutant enzyme is devoid of significant activity Escherichia coli
1.17.4.2 C647A mutant enzyme displays 2% of wild-type activity Escherichia coli
1.17.4.2 C662A mutant enzyme is devoid of significant activity Escherichia coli
1.17.4.2 C665A mutant enzyme is devoid of significant activity Escherichia coli

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.1.98.6 Zn C-terminal region contains a Zn(Cys)4 center. Residues Cys543, Cys546, Cys561, and Cys564 coordinate the metal ion tetrahedrally Tequatrovirus T4
1.1.98.6 Zn2+ C-terminal region contains a Zn(Cys)4 center Escherichia coli

Organism

EC Number Organism UniProt Comment Textmining
1.1.98.6 Escherichia coli P28903
-
-
1.1.98.6 Tequatrovirus T4 P07071
-
-
1.17.4.2 Escherichia coli
-
 JM109
-
1.17.4.2 Escherichia coli JM109
-
 JM109
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.17.4.2 wild-type and mutant enzymes Escherichia coli

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.17.4.2 CTP + reduced thioredoxin
-
 Escherichia coli dCTP + thioredoxin disulfide + H2O
-
 ?
1.17.4.2 CTP + reduced thioredoxin
-
 Escherichia coli JM109 dCTP + thioredoxin disulfide + H2O
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.17.4.2 class III RNR
-
 Escherichia coli