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Literature summary extracted from

  • Nyvall, P.; Pelloux, J.; Davies, H.V.; Pedersen, M.; Viola, R.
    Purification and characterization of a novel starch synthase selective for uridine 5'-diphosphate glucose from the red alga Gracilaria tenuistipitata (1999), Planta, 209, 143-152.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.4.1.242 citrate stimulates the UDP-glucose utilizing enzyme Agarophyton tenuistipitatum
2.4.1.242 UDP product inhibition of the UDP-glucose utilizing enzyme Agarophyton tenuistipitatum

General Stability

EC Number General Stability Organism
2.4.1.242 rapid loss of activity of purified UDP-glucose utilizing enzyme within hours Agarophyton tenuistipitatum

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.4.1.242 additional information UDP-glucose utilizing activity is sensitive to proteolytic inhibition during extraction, while the ADP-glucose utilizing activity is unaffected Agarophyton tenuistipitatum

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.4.1.242 additional information
-
 additional information kinetics Agarophyton tenuistipitatum
2.4.1.242 0.17
-
 glycogen UDP-glucose utilizing enzyme in presence of 0.5 M citrate Agarophyton tenuistipitatum
2.4.1.242 0.52
-
 amylopectin UDP-glucose utilizing enzyme in presence of 0.5 M citrate Agarophyton tenuistipitatum
2.4.1.242 0.69
-
 glycogen UDP-glucose utilizing enzyme in absence of citrate Agarophyton tenuistipitatum

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.4.1.242 cytosol starch granule-bound Agarophyton tenuistipitatum 5829
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.4.1.242 145000
-
 4 * 145000, UDP-glucose utilizing enzyme, SDS-PAGE Agarophyton tenuistipitatum
2.4.1.242 580000
-
 UDP-glucose utilizing enzyme, gel filtration Agarophyton tenuistipitatum

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.4.1.242 ADP-glucose + (1,4-alpha-glucosyl)n Agarophyton tenuistipitatum ADP-glucose is almost 9fold less effective than UDP-glucose, utilized by the ADP-glucose utilizing enzyme ADP + (1,4-alpha-glucosyl)n+1
-
 ?
2.4.1.242 UDP-glucose + (1,4-alpha-glucosyl)n Agarophyton tenuistipitatum UDP-glucose is the preferred glucosyl donor, utilized by the UDP-glucose utilizing enzyme UDP + (1,4-alpha-glucosyl)n+1
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.4.1.242 Agarophyton tenuistipitatum
-
 red alga
-

Oxidation Stability

EC Number Oxidation Stability Organism
2.4.1.242 UDP-glucose utilizing activity is sensitive to oxidative inhibition during extraction, while the ADP-glucose utilizing activity is unaffected Agarophyton tenuistipitatum

Purification (Commentary)

EC Number Purification (Comment) Organism
2.4.1.242 ADP-glucose utilizing activity partially, UDP-glucose utilizing activity 368.7fold to homogeneity Agarophyton tenuistipitatum

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.4.1.242 additional information
-
 UDP-glucose utilizing and ADP-glucose utilizing activities in different cell fractions and with different primer substrate, overview Agarophyton tenuistipitatum
2.4.1.242 0.229
-
 purified UDP-glucose utilizing enzyme Agarophyton tenuistipitatum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.1.242 ADP-glucose + (1,4-alpha-glucosyl)n ADP-glucose is almost 9fold less effective than UDP-glucose, utilized by the ADP-glucose utilizing enzyme Agarophyton tenuistipitatum ADP + (1,4-alpha-glucosyl)n+1
-
 ?
2.4.1.242 ADP-glucose + (1,4-alpha-glucosyl)n ADP-glucose is almost 9fold less effective than UDP-glucose, utilized by the ADP-glucose utilizing enzyme and UDP-glucose utilizing enzyme, the latter shows 3.8% activity compared to UDP-glucose Agarophyton tenuistipitatum ADP + (1,4-alpha-glucosyl)n+1
-
 ?
2.4.1.242 ADP-glucose + amylopectin
-
 Agarophyton tenuistipitatum ADP + ?
-
 ?
2.4.1.242 CDP-glucose + (1,4-alpha-glucosyl)n UDP-glucose utilizing enzyme, 2.5% of the activity with UDP-glucose Agarophyton tenuistipitatum CDP + ?
-
 ?
2.4.1.242 GDP-glucose + (1,4-alpha-glucosyl)n UDP-glucose utilizing enzyme, 28.7% of the activity with UDP-glucose Agarophyton tenuistipitatum GDP + ?
-
 ?
2.4.1.242 additional information the UDP-glucose utilizing enzyme shows broad primer specificity Agarophyton tenuistipitatum ?
-
 ?
2.4.1.242 NDP-glucose + glycogen
-
 Agarophyton tenuistipitatum ?
-
 ?
2.4.1.242 UDP-galactose + (1,4-alpha-glucosyl)n UDP-glucose utilizing enzyme, 10.1% of the activity with UDP-glucose Agarophyton tenuistipitatum UDP + ?
-
 ?
2.4.1.242 UDP-glucose + (1,4-alpha-glucosyl)n UDP-glucose is the preferred glucosyl donor, utilized by the UDP-glucose utilizing enzyme Agarophyton tenuistipitatum UDP + (1,4-alpha-glucosyl)n+1
-
 ?

Subunits

EC Number Subunits Comment Organism
2.4.1.242 tetramer 4 * 145000, UDP-glucose utilizing enzyme, SDS-PAGE Agarophyton tenuistipitatum

Synonyms

EC Number Synonyms Comment Organism
2.4.1.242 UDPGlc: starch synthase
-
 Agarophyton tenuistipitatum

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.4.1.242 6.7 8.1 with substrate glycogen Agarophyton tenuistipitatum
2.4.1.242 8.1
-
 with substrate amylopectin Agarophyton tenuistipitatum

pH Range

EC Number pH Minimum pH Maximum Comment Organism
2.4.1.242 6.5 8.5 with substrate amylopectin, sharp drop of activity below pH 6.5 and more gradually above pH 8.0 Agarophyton tenuistipitatum
2.4.1.242 7 9 with substrate amylopectin, sharp drop of activity below pH 7.0 and above pH 9.0 Agarophyton tenuistipitatum