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Literature summary extracted from

  • Sulova, Z.; Baran, R.; Farkas, V.
    Divergent modes of action on xyloglucan of two isoenzymes of xyloglucan endo-transglycosylase from Tropaeolum majus (2003), Plant Physiol. Biochem., 41, 431-437.
No PubMed abstract available

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.4.1.207 0.017
-
 rhodamin-conjugated Glc3-Xyl3-Gal2-glucitol isozyme eXET, pH 5.5, 30°C Tropaeolum majus
2.4.1.207 0.131
-
 rhodamin-conjugated Glc3-Xyl3-Gal2-glucitol isozyme sXET, pH 5.5, 30°C Tropaeolum majus

Organism

EC Number Organism UniProt Comment Textmining
2.4.1.207 Tropaeolum majus
-
 2 isoenzymes, one of which is seed specific
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.4.1.207 isozymes sXET and eXET from germinated seeds, i.e. cotyledons, and from epicotyls, respectively, to near homogeneity by ammonium sulfate fractionation, anion exchange chromatography, adsorption and desorption of the enzyme-substrate complex on cellulose, and hydrophobic-interaction chromatography Tropaeolum majus

Reaction

EC Number Reaction Comment Organism Reaction ID
2.4.1.207 2 Xylalpha(1-6)Glcbeta(1-4)(Xylalpha(1-6))Glcbeta(1-4))(Xylalpha(1-6))Glcbeta(1-4)(Xylalpha(1-6))Glcbeta(1-4)Glc = Xylalpha(1-6)Glcbeta(1-4)(Xylalpha(1-6))Glc + Xylalpha(1-6))Glcbeta(1-4)(Xylalpha(1-6))Glcbeta(1-4)Glcbeta(1-4)(Xylalpha(1-6))Glcbeta(1-4)(Xylalpha(1-6))Glcbeta(1-4))(Xylalpha(1-6))Glcbeta(1-4)(Xylalpha(1-6))Glcbeta(1-4)Glc determination of cleavage sites within the donor substrate of isozymes Tropaeolum majus
a

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.4.1.207 epicotyl isozyme eXET Tropaeolum majus
-
2.4.1.207 additional information isozyme eXET also occurs in other growing regions Tropaeolum majus
-
2.4.1.207 seed expression of isozyme sXET during germination Tropaeolum majus
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.1.207 additional information isozymes sXET and eXET show different specificities for xyloglucan-derived oligosaccharide acceptor substrates, and distinct preferences in selecting the site of attack on xyloglucan molecules Tropaeolum majus ?
-
 ?
2.4.1.207 rhodamin-conjugated Glc3-Xyl3-Gal2-glucitol + xyloglucan acceptor substrate: rhodamin-conjugated reduced xyloglucan-derived nonasaccharide, donor substrate: tamarind-seed xyloglucan, fluorescence assay Tropaeolum majus ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.4.1.207 XET
-
 Tropaeolum majus
2.4.1.207 xyloglucan endo-transglycosylase
-
 Tropaeolum majus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.4.1.207 30
-
 assay at Tropaeolum majus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.4.1.207 5 6 isozyme eXET Tropaeolum majus
2.4.1.207 5.5 8 isozyme sXET, broad optimum Tropaeolum majus

pH Range

EC Number pH Minimum pH Maximum Comment Organism
2.4.1.207 additional information
-
 isozymes sXET and eXET show different pH-profiles Tropaeolum majus

pI Value

EC Number Organism Comment pI Value Maximum pI Value
2.4.1.207 Tropaeolum majus about, isozymes sXET and eXET, isoelectric focusing
-
 6.6