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Literature summary extracted from

  • Turner, W.L.; Waller, J.C.; Vanderbeld, B.; Snedden, W.A.
    Cloning and characterization of two NAD kinases from Arabidopsis. identification of a calmodulin binding isoform (2004), Plant Physiol., 135, 1243-1255.
    View publication on PubMedView publication on EuropePMC

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.7.1.23 Ca2+
-
 Arabidopsis thaliana
2.7.1.23 Calmodulin
-
 Arabidopsis thaliana
2.7.1.23 Calmodulin calmodulin-dependent isoform, full activation in presence of Ca2+ Arabidopsis thaliana

Application

EC Number Application Comment Organism
2.7.1.23 analysis studies of enzyme activation by calmodulin, calmodulin-like proteins, calcium-binding proteins, and calmodulin mutants Arabidopsis thaliana

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.1.23
-
 Arabidopsis thaliana

General Stability

EC Number General Stability Organism
2.7.1.23 unstable Arabidopsis thaliana
2.7.1.23 unstable, loses activity at room temperature overnight Arabidopsis thaliana

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.1.23 Ca2+ 12% inhibition at 0.2 mM, 50% inhibition at 2 mM Arabidopsis thaliana
2.7.1.23 Trifluoperazine calmodulin-dependent isoform, 50% inhibition at 0.057 mM, isoforms NADK1, NADK2, no effect Arabidopsis thaliana

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.1.23 0.17
-
 ATP pH 7.9, 25°C Arabidopsis thaliana
2.7.1.23 0.17
-
 ATP calmodulin-dependent isoform, pH 7.9, 25°C Arabidopsis thaliana
2.7.1.23 0.17
-
 ATP ATP in form of MgATP2- Arabidopsis thaliana
2.7.1.23 0.2
-
 NAD+ pH 7.9, 25°C Arabidopsis thaliana
2.7.1.23 0.2
-
 NAD+ calmodulin-dependent isoform, pH 7.9, 25°C Arabidopsis thaliana
2.7.1.23 0.43
-
 NAD+ pH 7.9, 25°C Arabidopsis thaliana
2.7.1.23 0.43
-
 NAD+ isoform NADK2, pH 7.9, 25°C Arabidopsis thaliana
2.7.1.23 0.52
-
 NAD+ pH 7.9, 25°C Arabidopsis thaliana
2.7.1.23 0.52
-
 NAD+ isoform NADK1, pH 7.9, 25°C Arabidopsis thaliana
2.7.1.23 0.73
-
 ATP pH 7.9, 25°C Arabidopsis thaliana
2.7.1.23 0.73
-
 ATP isoform NADK1, pH 7.9, 25°C Arabidopsis thaliana
2.7.1.23 0.73
-
 ATP ATP in form of MgATP2- Arabidopsis thaliana
2.7.1.23 0.74
-
 ATP pH 7.9, 25°C Arabidopsis thaliana
2.7.1.23 0.74
-
 ATP isoform NADK2, pH 7.9, 25°C Arabidopsis thaliana
2.7.1.23 0.74
-
 ATP ATP in form of MgATP2- Arabidopsis thaliana

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.7.1.23 chloroplast putative Arabidopsis thaliana 9507
-
2.7.1.23 cytosol putative Arabidopsis thaliana 5829
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.1.23 Mg2+
-
 Arabidopsis thaliana
2.7.1.23 Mg2+ required to stabilize phosphoanhydride bonds to ATP Arabidopsis thaliana

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.1.23 58200
-
-
 Arabidopsis thaliana
2.7.1.23 109200
-
-
 Arabidopsis thaliana

Organism

EC Number Organism UniProt Comment Textmining
2.7.1.23 Arabidopsis thaliana
-
-
-
2.7.1.23 Arabidopsis thaliana
-
 known to be present in other plants
-
2.7.1.23 Arabidopsis thaliana
-
 isoforms NADK1, NADK2 plus a calmodulin-dependent isoform isolated from seedling
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.1.23 partial Arabidopsis thaliana
2.7.1.23 recombinant isoforms NADK1, NADK2, partial purification of calmodulin-dependent isoform Arabidopsis thaliana
2.7.1.23 single-step calmodulin-affinity chromatography Arabidopsis thaliana

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.7.1.23 additional information both isoforms NADK1 and NADK2 are present in all tissues examined and throughout development Arabidopsis thaliana
-
2.7.1.23 seedling calmodulin-dependent isoform Arabidopsis thaliana
-
2.7.1.23 whole plant
-
 Arabidopsis thaliana
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.7.1.23 11.1
-
 pH 7.9, 25°C Arabidopsis thaliana
2.7.1.23 11.1
-
 isoform NADK1, pH 7.9, 25°C Arabidopsis thaliana
2.7.1.23 14.3
-
 pH 7.9, 25°C Arabidopsis thaliana
2.7.1.23 14.3
-
 isoform NADK2, pH 7.9, 25°C Arabidopsis thaliana
2.7.1.23 213
-
-
 Arabidopsis thaliana
2.7.1.23 213
-
 calmodulin-depndent isoform, pH 7.9, 25°C Arabidopsis thaliana

Storage Stability

EC Number Storage Stability Organism
2.7.1.23 unstable Arabidopsis thaliana

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.1.23 ATP + NAD+
-
 Arabidopsis thaliana ADP + NADP+
-
 ?
2.7.1.23 ATP + NAD+ UTP also may be used as phosphoryl donor Arabidopsis thaliana ADP + NADP+
-
 ?
2.7.1.23 additional information NADP is not phosphorylated, tetrapolyphosphate or diphosphate are not used as phosphoryl donors Arabidopsis thaliana ?
-
 ?

Subunits

EC Number Subunits Comment Organism
2.7.1.23 More Ca2+-dependent calmodulin-binding domain near N-terminal extension of isoform NADK2 Arabidopsis thaliana

Synonyms

EC Number Synonyms Comment Organism
2.7.1.23 diphosphopyridine nucleotide kinase
-
 Arabidopsis thaliana
2.7.1.23 diphosphppyridine kinase
-
 Arabidopsis thaliana
2.7.1.23 NADK1 calcium-calmodulin nonbinding isoform of plant NAD+ kinase Arabidopsis thaliana
2.7.1.23 NADK2 calcium- and calmodulin-binding isoform of plant NAD+ kinase Arabidopsis thaliana
2.7.1.23 native plant calcium- and calmodulin-dependent NAD+-kinase
-
 Arabidopsis thaliana

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.1.23 7.9
-
-
 Arabidopsis thaliana
2.7.1.23 7.9
-
 isoforms NADK1, NADK2 Arabidopsis thaliana
2.7.1.23 8
-
-
 Arabidopsis thaliana
2.7.1.23 8
-
 calmodulin-dependent isoform Arabidopsis thaliana

pH Range

EC Number pH Minimum pH Maximum Comment Organism
2.7.1.23 additional information
-
 broad Arabidopsis thaliana
2.7.1.23 7.9
-
-
 Arabidopsis thaliana