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Literature summary extracted from

  • Sivak, M.N.; Wagner, M.; Preiss, J.
    Biochemical evidence for the role of the waxy protein from pea (Pisum sativum L.) as a granule-bound starch synthase (1993), Plant Physiol., 103, 1355-1359.
    View publication on PubMedView publication on EuropePMC

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.4.1.242 58000 60000 sucrose density gradient centrifugation Pisum sativum
2.4.1.242 60000
-
 1 * 60000, SDS-PAGE Pisum sativum

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.4.1.242 ADP-glucose + (1,4-alpha-glucosyl)n Pisum sativum the Waxy protein and the granule-bound starch synthase are identical ADP + (1,4-alpha-glucosyl)n+1
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.4.1.242 Pisum sativum
-
 round-seeded var. Alaska, 2 isozymes
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.4.1.242 partially from lyophilized starch extracted from developing embryos, by anion exchange chromatography Pisum sativum

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.4.1.242 embryo developing Pisum sativum
-
2.4.1.242 seed starch granule Pisum sativum
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.4.1.242 10
-
 partially purified isozyme I Pisum sativum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.1.242 ADP-glucose + (1,4-alpha-glucosyl)n
-
 Pisum sativum ADP + (1,4-alpha-glucosyl)n+1
-
 ?
2.4.1.242 ADP-glucose + (1,4-alpha-glucosyl)n the Waxy protein and the granule-bound starch synthase are identical Pisum sativum ADP + (1,4-alpha-glucosyl)n+1
-
 ?

Subunits

EC Number Subunits Comment Organism
2.4.1.242 monomer 1 * 60000, SDS-PAGE Pisum sativum

Synonyms

EC Number Synonyms Comment Organism
2.4.1.242 GBSSI
-
 Pisum sativum
2.4.1.242 granule-bound starch synthase
-
 Pisum sativum
2.4.1.242 Waxy protein
-
 Pisum sativum

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.4.1.242 30
-
 assay at Pisum sativum

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.4.1.242 8.4
-
 assay at Pisum sativum