EC Number | Cloned (Comment) | Organism |
---|---|---|
2.5.1.112 | expressed in Escherichia coli AD494(DE3)pLysS cells | Arabidopsis thaliana |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.5.1.27 | 0.0065 | - |
dimethylallyl diphosphate | AtIPT4 | Arabidopsis thaliana | |
2.5.1.27 | 0.018 | - |
ATP | AtIPT4 | Arabidopsis thaliana | |
2.5.1.112 | 0.0065 | - |
dimethylallyl diphosphate | in 25 mM Tris-HCl, pH 7.5, 10 mM MgCl2+, 5 mM 2-mercaptoethanol at 24°C | Arabidopsis thaliana | |
2.5.1.112 | 0.018 | - |
ATP | in 25 mM Tris-HCl, pH 7.5, 10 mM MgCl2+, 5 mM 2-mercaptoethanol at 24°C | Arabidopsis thaliana |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.5.1.112 | dimethylallyl diphosphate + ADP | Arabidopsis thaliana | - |
diphosphate + N6-(dimethylallyl)adenosine 5'-diphosphate | - |
? | |
2.5.1.112 | dimethylallyl diphosphate + ATP | Arabidopsis thaliana | highest activity with ATP | diphosphate + N6-(dimethylallyl)adenosine 5'-triphosphate | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
2.5.1.27 | Arabidopsis thaliana | Q94ID3 | AtIPT1 | - |
2.5.1.27 | Arabidopsis thaliana | Q94ID3 | AtIPT4 | - |
2.5.1.112 | Arabidopsis thaliana | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
2.5.1.112 | Ni-NTA agarose column chromatography | Arabidopsis thaliana |
EC Number | Source Tissue | Comment | Organism | Textmining |
---|---|---|---|---|
2.5.1.112 | callus | - |
Arabidopsis thaliana | - |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.5.1.27 | dimethylallyl diphosphate + ADP | the enzyme preferrs ADP and ATP rather than AMP | Arabidopsis thaliana | dimethylallyl-ADP + diphosphate | - |
? | |
2.5.1.27 | dimethylallyl diphosphate + AMP | the enzyme preferrs ADP and ATP rather than AMP | Arabidopsis thaliana | diphosphate + N6-(dimethylallyl)adenosine 5'-phosphate | - |
? | |
2.5.1.27 | dimethylallyl diphosphate + ATP | the enzyme preferrs ADP and ATP rather than AMP | Arabidopsis thaliana | dimethylallyl-ATP + diphosphate | - |
? | |
2.5.1.112 | dimethylallyl diphosphate + ADP | - |
Arabidopsis thaliana | diphosphate + N6-(dimethylallyl)adenosine 5'-diphosphate | - |
? | |
2.5.1.112 | dimethylallyl diphosphate + ATP | highest activity with ATP | Arabidopsis thaliana | diphosphate + N6-(dimethylallyl)adenosine 5'-triphosphate | - |
? | |
2.5.1.112 | additional information | no activity with AMP and tRNA | Arabidopsis thaliana | ? | - |
? |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
2.5.1.27 | AtIPT1 | - |
Arabidopsis thaliana |
2.5.1.27 | AtIPT4 | - |
Arabidopsis thaliana |
2.5.1.27 | DMAPP:ATP/ADP isppentenyltransferase | - |
Arabidopsis thaliana |
2.5.1.112 | dimethylallyl diphosphate:ATP/ADP isopentenyltransferase | - |
Arabidopsis thaliana |
2.5.1.112 | IPT4 | - |
Arabidopsis thaliana |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
2.5.1.112 | ADP | - |
Arabidopsis thaliana | |
2.5.1.112 | ATP | - |
Arabidopsis thaliana |