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Literature summary extracted from

  • Hübner, S.; Hehmann, M.; Schreiner, S.; Martens, S.; Lukacin, R.; Matern, U.
    Functional expression of cinnamate 4-hydroxylase from Ammi majus L (2003), Phytochemistry, 64, 445-452.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.14.14.91 expression in microsomes of yeast strain INVSc1 Ammi majus

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.14.14.91 8-methoxypsoralen
-
 Ammi majus
1.14.14.91 psoralen
-
 Ammi majus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.14.14.91 0.0089
-
 trans-cinnamate
-
 Ammi majus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.14.14.91 trans-cinnamate + [reduced NADPH-hemoprotein reductase] + O2 Ammi majus first step in the formation of coumarin derivatives 4-hydroxycinnamate + [oxidized NADPH-hemoprotein reductase] + H2O
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.14.14.91 Ammi majus
-
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.14.14.91 cell suspension culture
-
 Ammi majus
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.14.91 trans-cinnamate + [reduced NADPH-hemoprotein reductase] + O2
-
 Ammi majus 4-hydroxycinnamate + [oxidized NADPH-hemoprotein reductase] + H2O
-
 ?
1.14.14.91 trans-cinnamate + [reduced NADPH-hemoprotein reductase] + O2 first step in the formation of coumarin derivatives Ammi majus 4-hydroxycinnamate + [oxidized NADPH-hemoprotein reductase] + H2O
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.14.14.91 25
-
-
 Ammi majus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.14.14.91 6.5 7
-
 Ammi majus