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Literature summary extracted from

  • Rubio, L.M.; Flores, E.; Herrero, A.
    Purification, cofactor analysis, and site-directed mutagenesis of Synechococcus ferredoxin-nitrate reductase (2002), Photosynth. Res., 72, 13-26.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.7.7.2 expression in Escherichia coli Synechococcus sp.

Protein Variants

EC Number Protein Variants Comment Organism
1.7.7.2 C12A no actiivty, no iron-sulfur cluster Synechococcus sp.
1.7.7.2 C16S 1.9% of wild type activity with ferredoxin, no iron-sulfur cluster Synechococcus sp.
1.7.7.2 C56A no activity, no iron-sulfur cluster Synechococcus sp.
1.7.7.2 C56S 70% of wild type activity with ferredoxin, contains an iron-sulfur cluster Synechococcus sp.
1.7.7.2 C9A no activity, no iron-sulfur cluster Synechococcus sp.
1.7.7.2 C9H no narB expression Synechococcus sp.

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.7.7.2 Iron enzyme contains 1 [3Fe-4S] cluster Synechococcus sp.
1.7.7.2 Molybdenum 1 atom of Mo and 2 molecules of ribonucleotide-conjugated molybdopterin per molecule of protein Synechococcus sp.

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.7.7.2 nitrate + reduced ferredoxin Synechococcus sp.
-
 nitrite + H2O + oxidized ferredoxin
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.7.7.2 Synechococcus sp. P39458 strain PCC 7942
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.7.7.2 recombinant enzyme Synechococcus sp.

Storage Stability

EC Number Storage Stability Organism
1.7.7.2 -20°C, 20% glycerol, several months, no loss of activity Synechococcus sp.
1.7.7.2 4°C, several weeks, no loss of activity Synechococcus sp.

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.7.7.2 nitrate + reduced ferredoxin
-
 Synechococcus sp. nitrite + H2O + oxidized ferredoxin
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.7.7.2 narB
-
 Synechococcus sp.

Cofactor

EC Number Cofactor Comment Organism Structure
1.7.7.2 molybdopterin bis-molybdopterin guanine dinucleotide Synechococcus sp.