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Literature summary extracted from

  • De Gregorio, A.; Battistutta, R.; Arena, N.; Panzalorto, M.; Francescato, P.; Valentini, G.; Bruno, G.; Zanotti, G.
    Functional and structural characterization of ovine ornithine transcarbamoylase (2003), Org. Biomol. Chem., 1, 3178-3185.
    View publication on PubMed

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.1.3.3 0.033
-
 Carbamoylphosphate 37°C, pH 8.6 Ovis aries
2.1.3.3 0.087
-
 Carbamoylphosphate 37°C, pH 8.2 Ovis aries
2.1.3.3 0.25
-
 Carbamoylphosphate 37°C, pH 9.0 Ovis aries
2.1.3.3 0.25
-
 L-ornithine 37°C, pH 8.6 Ovis aries
2.1.3.3 0.33
-
 L-ornithine 37°C, pH 8.2 Ovis aries
2.1.3.3 0.45
-
 L-ornithine 37°C, pH 9.0 Ovis aries

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.1.3.3 34000
-
 3 * 34000, SDS-PAGE Ovis aries
2.1.3.3 102000
-
 gel filtration Ovis aries

Organism

EC Number Organism UniProt Comment Textmining
2.1.3.3 Ovis aries
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.1.3.3
-
 Ovis aries

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.1.3.3 liver
-
 Ovis aries
-

Storage Stability

EC Number Storage Stability Organism
2.1.3.3 -20°C, 25 mM sodium phosphate, 10 mM carbamoylphosphate, 25 mM norvalien, pH 7.0, stable for months Ovis aries

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.1.3.3 carbamoyl phosphate + L-ornithine
-
 Ovis aries phosphate + L-citrulline
-
 ?

Subunits

EC Number Subunits Comment Organism
2.1.3.3 trimer 3 * 34000, SDS-PAGE Ovis aries

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.1.3.3 50
-
 41 min, 50% residual activity Ovis aries
2.1.3.3 55
-
 16 min, 50% residual activity Ovis aries
2.1.3.3 63
-
 presence of 20 mM carbamoylphosphate plus 20 mM L-norvaline, stable Ovis aries
2.1.3.3 63
-
 presence of 20 mM carbamoylphosphate, 15 min, 50% residual activity Ovis aries
2.1.3.3 63
-
 presence of 20 mM ornithine, 3 min, 50% residual activity Ovis aries

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.1.3.3 8.2
-
 at 10 mM ornithine Ovis aries
2.1.3.3 8.6
-
 at 0.025 mM ornithine Ovis aries