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Literature summary extracted from

  • Yamamoto, T.; Maruta, K.; Mukai, K.; Yamashita, H.; Nishimoto, T.; Kubota, M.; Fukuda, S.; Kurimoto, M.; Tsujisaka, Y.
    Cloning and sequencing of kojibiose phosphorylase gene from Thermoanaerobacter brockii ATCC35047 (2004), J. Biosci. Bioeng., 98, 99-106.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.4.1.230
-
 Thermoanaerobacter brockii

Protein Variants

EC Number Protein Variants Comment Organism
2.4.1.230 D177N 106% of wild-type activity Thermoanaerobacter brockii
2.4.1.230 D271N 69% of wild-type activity Thermoanaerobacter brockii
2.4.1.230 D340N 89% of wild-type activity Thermoanaerobacter brockii
2.4.1.230 D362N complete loss of enzymic activity Thermoanaerobacter brockii
2.4.1.230 D459N increase in Km-values for kojibiose, beta-D-glucose 1-phosphate and glucose Thermoanaerobacter brockii
2.4.1.230 E284Q 103% of wild-type activity Thermoanaerobacter brockii
2.4.1.230 E642Q complete loss of enzymic activity Thermoanaerobacter brockii
2.4.1.230 K114Q 85% of wild-type activity Thermoanaerobacter brockii
2.4.1.230 K403Q 73% of wild-type activity Thermoanaerobacter brockii
2.4.1.230 K614Q complete loss of enzymic activity Thermoanaerobacter brockii
2.4.1.230 K749Q 78% of wild-type activity Thermoanaerobacter brockii
2.4.1.230 R137Q 61% of wild-type activity Thermoanaerobacter brockii
2.4.1.230 R33Q 31% of wild-type activity Thermoanaerobacter brockii
2.4.1.230 R476Q 50% of wild-type activity Thermoanaerobacter brockii
2.4.1.230 R48Q 62% of wild-type activity Thermoanaerobacter brockii

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.4.1.230 0.75
-
 phosphate wild-type, pH 5.5, 60°C Thermoanaerobacter brockii
2.4.1.230 0.79
-
 beta-D-glucose 1-phosphate wild-type, pH 5.5, 60°C Thermoanaerobacter brockii
2.4.1.230 0.8
-
 kojibiose wild-type, pH 5.5, 60°C Thermoanaerobacter brockii
2.4.1.230 1.11
-
 phosphate mutant D459N, pH 5.5, 60°C Thermoanaerobacter brockii
2.4.1.230 3.84
-
 beta-D-glucose wild-type, pH 5.5, 60°C Thermoanaerobacter brockii
2.4.1.230 5.21
-
 kojibiose mutant D459N, pH 5.5, 60°C Thermoanaerobacter brockii
2.4.1.230 8.57
-
 beta-D-glucose 1-phosphate mutant D459N, pH 5.5, 60°C Thermoanaerobacter brockii
2.4.1.230 25.4
-
 beta-D-glucose mutant D459N, pH 5.5, 60°C Thermoanaerobacter brockii

Organism

EC Number Organism UniProt Comment Textmining
2.4.1.230 Thermoanaerobacter brockii
-
 strain ATCC35047, expression in Escherichia coli
-
2.4.1.230 Thermoanaerobacter brockii ATCC 35047
-
 strain ATCC35047, expression in Escherichia coli
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.1.230 beta-D-glucose + beta-D-glucose 1-phosphate
-
 Thermoanaerobacter brockii 2-alpha-D-glucosyl-D-glucose + phosphate
-
 r
2.4.1.230 beta-D-glucose + beta-D-glucose 1-phosphate
-
 Thermoanaerobacter brockii ATCC 35047 2-alpha-D-glucosyl-D-glucose + phosphate
-
 r
2.4.1.230 kojibiose + phosphate
-
 Thermoanaerobacter brockii beta-D-glucose-1-phosphate + D-glucose
-
 r
2.4.1.230 kojibiose + phosphate
-
 Thermoanaerobacter brockii ATCC 35047 beta-D-glucose-1-phosphate + D-glucose
-
 r

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.4.1.230 65
-
-
 Thermoanaerobacter brockii

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.4.1.230 5.5
-
-
 Thermoanaerobacter brockii