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Literature summary extracted from

  • Tiainen, P.; Myllyharju, J.; Koivunen, P.
    Characterization of a second Arabidopsis thaliana prolyl 4-hydroxylase with distinct substrate specificity (2005), J. Biol. Chem., 280, 1142-1148.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.14.11.2 expression in Sf9 cells Arabidopsis thaliana

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.14.11.2 Pyridine-2,4-dicarboxylate competitive inhibition Arabidopsis thaliana
1.14.11.2 Pyridine-2,5-dicarboxylate competitive inhibition Arabidopsis thaliana

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.14.11.2 0.005
-
 Fe2+
-
 Arabidopsis thaliana
1.14.11.2 0.03
-
 poly L-proline molecular weight 5000-10000 Da Arabidopsis thaliana
1.14.11.2 0.13
-
 poly L-proline molecular weight 30000-40000 Da Arabidopsis thaliana
1.14.11.2 0.17
-
 2-oxoglutarate
-
 Arabidopsis thaliana
1.14.11.2 0.3
-
 ascorbate
-
 Arabidopsis thaliana
1.14.11.2 0.38
-
 SPPPPVYKSPPPPVKHYSPPPV sequence present in extensin Arabidopsis thaliana
1.14.11.2 0.66
-
 SPPPPVSPPPVSPPPPV sequence present in extensin Arabidopsis thaliana
1.14.11.2 1.26
-
 (ATPPPV)3 sequence present in arabinogalactan protein Arabidopsis thaliana

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.14.11.2 35000
-
 1 * 35000, SDS-PAGE Arabidopsis thaliana

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.14.11.2 procollagen L-proline + 2-oxoglutarate + O2 Arabidopsis thaliana
-
 procollagen trans-4-hydroxy-L-proline + succinate + CO2
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.14.11.2 Arabidopsis thaliana
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.11.2 (ATPPPV)3 + 2-oxoglutarate + O2
-
 Arabidopsis thaliana trans-4-hydroxy-L-proline (ATPPPV)3 + succinate + CO2
-
 ?
1.14.11.2 2-oxoglutarate + O2 + ascorbate
-
 Arabidopsis thaliana succinate + dehydroascorbate + CO2 + H2O
-
 ?
1.14.11.2 poly L-proline + 2-oxoglutarate + O2 molecular weights of 5000 Da, 8000 Da and 32000 Da, respectively Arabidopsis thaliana poly trans-4-hydroxy-L-proline + succinate + CO2
-
 ?
1.14.11.2 procollagen L-proline + 2-oxoglutarate + O2
-
 Arabidopsis thaliana procollagen trans-4-hydroxy-L-proline + succinate + CO2
-
 ?
1.14.11.2 SPPPPVSPPPVSPPPPV + 2-oxoglutarate + O2
-
 Arabidopsis thaliana trans-4-hydroxy-L-proline SPPPPVSPPPVSPPPPV + succinate + CO2
-
 ?
1.14.11.2 SPPPPVYKSPPPPVKHYSPPPV + 2-oxoglutarate + O2
-
 Arabidopsis thaliana trans-4-hydroxy-L-proline SPPPPVYKSPPPPVKHYSPPPV + succinate + CO2
-
 ?

Subunits

EC Number Subunits Comment Organism
1.14.11.2 monomer 1 * 35000, SDS-PAGE Arabidopsis thaliana

Synonyms

EC Number Synonyms Comment Organism
1.14.11.2 At-P4H-2
-
 Arabidopsis thaliana

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
1.14.11.2 0.01
-
 Pyridine-2,4-dicarboxylate
-
 Arabidopsis thaliana
1.14.11.2 0.08
-
 Pyridine-2,5-dicarboxylate
-
 Arabidopsis thaliana