Literature summary extracted from
Yasuno, R.; von Wettstein-Knowles, P.; Wada, H.
Identification and molecular characterization of the beta-ketoacyl-[acyl carrier protein] synthase component of the Arabidopsis mitochondrial fatty acid synthase (2004), J. Biol. Chem., 279, 8242-8251.
Cloned(Commentary)
EC Number |
Cloned (Comment) |
Organism |
---|
2.3.1.41 |
complements KAS II defect in Escherichia coli |
Arabidopsis thaliana |
Inhibitors
EC Number |
Inhibitors |
Comment |
Organism |
Structure |
---|
2.3.1.41 |
cerulenin |
- |
Arabidopsis thaliana |
|
Localization
EC Number |
Localization |
Comment |
Organism |
GeneOntology No. |
Textmining |
---|
2.3.1.41 |
mitochondrion |
- |
Arabidopsis thaliana |
5739 |
- |
Molecular Weight [Da]
EC Number |
Molecular Weight [Da] |
Molecular Weight Maximum [Da] |
Comment |
Organism |
---|
2.3.1.41 |
47000 |
- |
x * 47000, SDS-PAGE |
Arabidopsis thaliana |
Organism
EC Number |
Organism |
UniProt |
Comment |
Textmining |
---|
2.3.1.41 |
Arabidopsis thaliana |
Q8L3X9 |
expression in Escherichia coli |
- |
Posttranslational Modification
EC Number |
Posttranslational Modification |
Comment |
Organism |
---|
2.3.1.41 |
proteolytic modification |
20 amino acid mitochondrial targeting sequence |
Arabidopsis thaliana |
Purification (Commentary)
EC Number |
Purification (Comment) |
Organism |
---|
2.3.1.41 |
recombinant enzyme |
Arabidopsis thaliana |
Specific Activity [micromol/min/mg]
EC Number |
Specific Activity Minimum [µmol/min/mg] |
Specific Activity Maximum [µmol/min/mg] |
Comment |
Organism |
---|
2.3.1.41 |
additional information |
- |
enzyme from fresh extract synthesizes prominent amounts of C14 and C16 acyl chains. After storage at -20°C for one or several weeks, enzyme synthesizes predominantly C8 acyl chains |
Arabidopsis thaliana |
Storage Stability
EC Number |
Storage Stability |
Organism |
---|
2.3.1.41 |
0°C, unstable in crude extracts lacking glycerol. Enzyme from fresh extract synthesizes prominent amounts of C14 and C16 acyl chains. After storage at -20°C for one or several weeks, enzyme synthesizes predominantly C8 acyl chains |
Arabidopsis thaliana |
Substrates and Products (Substrate)
EC Number |
Substrates |
Comment Substrates |
Organism |
Products |
Comment (Products) |
Rev. |
Reac. |
---|
2.3.1.41 |
acyl-[acyl-carrier protein] + malonyl-[acyl-carrier protein] |
- |
Arabidopsis thaliana |
3-oxoacyl-[acyl-carrier protein] + CO2 + [acyl-carrier protein] |
- |
? |
|
2.3.1.41 |
additional information |
no substrate: acetyl-[acyl-carrier protein] |
Arabidopsis thaliana |
? |
- |
? |
|
Subunits
EC Number |
Subunits |
Comment |
Organism |
---|
2.3.1.41 |
? |
x * 47000, SDS-PAGE |
Arabidopsis thaliana |