Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Kataoka, K.; Yamaguchi, K.; Kobayashi, M.; Mori, T.; Bokui, N.; Suzuki, S.
    Structure-based engineering of Alcaligenes xylosoxidans copper-containing nitrite reductase enhances intermolecular electron transfer reaction with pseudoazurin (2004), J. Biol. Chem., 279, 53374-53378.
    View publication on PubMed

Protein Variants

EC Number Protein Variants Comment Organism
1.7.2.1 A191E slight increase in electron transfer rate constant Achromobacter xylosoxidans
1.7.2.1 A191E/G198E 3fold increase in electron transfer rate constant Achromobacter xylosoxidans
1.7.2.1 A83D slight increase in electron transfer rate constant Achromobacter xylosoxidans
1.7.2.1 A83D/A191E 3fold increase in electron transfer rate constant Achromobacter xylosoxidans
1.7.2.1 A83D/A191E/G198E 4.7fold increase in electron transfer rate constant Achromobacter xylosoxidans
1.7.2.1 A83D/G198E 3fold increase in electron transfer rate constant Achromobacter xylosoxidans
1.7.2.1 G198E 2.6fold increase in electron transfer rate constant Achromobacter xylosoxidans

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.7.2.1 0.00008
-
 nitrite 25°C, A83D/A191E/G198E triple mutant Achromobacter xylosoxidans
1.7.2.1 0.00024
-
 nitrite 25°C, A191E/G198E double mutant Achromobacter xylosoxidans
1.7.2.1 0.00025
-
 nitrite 25°C, A83D/G198E double mutant Achromobacter xylosoxidans
1.7.2.1 0.0003
-
 nitrite 25°C, A83D/A191E double mutant Achromobacter xylosoxidans
1.7.2.1 0.0005
-
 nitrite 25°C, G198E mutant Achromobacter xylosoxidans
1.7.2.1 0.00096
-
 nitrite 25°C, A191E mutant Achromobacter xylosoxidans
1.7.2.1 0.001
-
 nitrite 25°C, A83D mutant Achromobacter xylosoxidans
1.7.2.1 0.0013
-
 nitrite 25°C, wild-type Achromobacter xylosoxidans

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.7.2.1 copper copper-containing nitrite reductase Achromobacter xylosoxidans

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.7.2.1 nitrite + reduced pseudoazurin Achromobacter xylosoxidans
-
 NO + oxidized pseudoazurin
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.7.2.1 Achromobacter xylosoxidans
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.7.2.1 nitrite + reduced pseudoazurin
-
 Achromobacter xylosoxidans NO + oxidized pseudoazurin
-
 ?
1.7.2.1 nitrite + reduced pseudoazurin pseudoazurin from Achromobacter cycloclastes Achromobacter xylosoxidans NO + oxidized pseudoazurin
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.7.2.1 AxNiR
-
 Achromobacter xylosoxidans

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.7.2.1 172
-
 nitrite 25°C Achromobacter xylosoxidans