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Literature summary extracted from

  • Cabello, P.; Pino, C.; Olmo-Mira, M.F.; Castillo, F.; Roldan, M.D.; Moreno-Vivian, C.
    Hydroxylamine assimilation by Rhodobacter capsulatus E1F1. Requirement of the hcp gene (hybrid cluster protein) located in the nitrate assimilation nas gene region for hydroxylamine reduction (2004), J. Biol. Chem., 279, 45485-45494.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.7.1.10 expressed in Escherichia coli JM109 as His-tag fusion protein Rhodobacter capsulatus

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.7.1.10 2,2'-dipyridyl
-
 Rhodobacter capsulatus
1.7.1.10 CN-
-
 Rhodobacter capsulatus
1.7.1.10 dithioerythritol
-
 Rhodobacter capsulatus
1.7.1.10 o-phenanthroline strong inhibition Rhodobacter capsulatus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.7.1.10 0.007
-
 methyl viologen pH 9.3, 40°C Rhodobacter capsulatus
1.7.1.10 1
-
 hydroxylamine pH 9.3, 40°C Rhodobacter capsulatus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.7.1.10 53000
-
 SDS-PAGE Rhodobacter capsulatus
1.7.1.10 57800
-
 calculated from the deduced amino acid sequence Rhodobacter capsulatus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.7.1.10 hydroxylamine + NADH Rhodobacter capsulatus involved in the assimilation of toxic hydroxylamine formed during nitrate assimilation NH3 + NAD+ + H2O
-
 ?
1.7.1.10 hydroxylamine + NADH Rhodobacter capsulatus E1F1 involved in the assimilation of toxic hydroxylamine formed during nitrate assimilation NH3 + NAD+ + H2O
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.7.1.10 Rhodobacter capsulatus
-
 highest activity in cells grown in the presence of hydroxylamine
-
1.7.1.10 Rhodobacter capsulatus E1F1
-
 highest activity in cells grown in the presence of hydroxylamine
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.7.1.10 recombinant protein using His-tag Rhodobacter capsulatus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.7.1.10 hydroxylamine + methyl viologen
-
 Rhodobacter capsulatus NH3 + H2O + ?
-
 ?
1.7.1.10 hydroxylamine + methyl viologen
-
 Rhodobacter capsulatus E1F1 NH3 + H2O + ?
-
 ?
1.7.1.10 hydroxylamine + NADH involved in the assimilation of toxic hydroxylamine formed during nitrate assimilation Rhodobacter capsulatus NH3 + NAD+ + H2O
-
 ?
1.7.1.10 hydroxylamine + NADH involved in the assimilation of toxic hydroxylamine formed during nitrate assimilation Rhodobacter capsulatus E1F1 NH3 + NAD+ + H2O
-
 ?
1.7.1.10 hydroxylamine + NADH only native, not recombinant protein, recombinant protein lacks NADH oxidoreductase activity Rhodobacter capsulatus NH3 + H2O + NAD+
-
 ?
1.7.1.10 hydroxylamine + NADH only native, not recombinant protein, recombinant protein lacks NADH oxidoreductase activity Rhodobacter capsulatus E1F1 NH3 + H2O + NAD+
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.7.1.10 hybrid cluster protein formerly primane protein, displays hydroxylamine reductase activity Rhodobacter capsulatus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.7.1.10 40
-
-
 Rhodobacter capsulatus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.7.1.10 9.3
-
-
 Rhodobacter capsulatus

Cofactor

EC Number Cofactor Comment Organism Structure
1.7.1.10 iron-sulfur centre [2Fe-2S] and [4Fe-2S-2O] Rhodobacter capsulatus
1.7.1.10 NADH
-
 Rhodobacter capsulatus