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Literature summary extracted from

  • Ishimizu, T.; Sasaki, A.; Okutani, S.; Maeda, M.; Yamagishi, M.; Hase, S.
    Endo-beta-mannosidase, a plant enzyme acting on N-glycan: purification, molecular cloning, and characterization (2004), J. Biol. Chem., 279, 38555-38562.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.2.1.152 expressed in Escherichia coli RosettaBlue Arabidopsis thaliana

Protein Variants

EC Number Protein Variants Comment Organism
3.2.1.152 E464A no activity Arabidopsis thaliana
3.2.1.152 E549A no activity Arabidopsis thaliana

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.152 78000
-
 gel filtration Lilium longiflorum
3.2.1.152 112000
-
-
 Arabidopsis thaliana

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.2.1.152 Man-beta-(1-4)GlcNAc + H2O Lilium longiflorum hydrolysis of Man-beta-(1-4)GlcNAc linkage in the trimannosyl core structure of N-glycans ?
-
 ?
3.2.1.152 Man-beta-(1-4)GlcNAc + H2O Arabidopsis thaliana hydrolysis of Man-beta-(1-4)GlcNAc linkage in the trimannosyl core structure of N-glycans ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.152 Arabidopsis thaliana Q75W54
-
-
3.2.1.152 Lilium longiflorum
-
 Thunb. cv Hinomoto
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.152
-
 Lilium longiflorum
3.2.1.152 partial purification of the recombinant protein Arabidopsis thaliana

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.2.1.152 flower
-
 Lilium longiflorum
-
3.2.1.152 flower
-
 Arabidopsis thaliana
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.152 0.026
-
 purified enzyme, pH 5.0, 37°C Arabidopsis thaliana

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.152 Man-alpha-(1-6)Man-beta-(1-4)GlcNAc-beta-(1-4)GlcNAc-pyridylamine + H2O
-
 Lilium longiflorum GlcNAc-beta-(1-4)GlcNAc-pyridylamine + Man-alpha-(1-6)Man
-
 ?
3.2.1.152 Man-alpha-(1-6)Man-beta-(1-4)GlcNAc-beta-(1-4)GlcNAc-pyridylamine + H2O
-
 Arabidopsis thaliana GlcNAc-beta-(1-4)GlcNAc-pyridylamine + Man-alpha-(1-6)Man
-
 ?
3.2.1.152 Man-beta-(1-4)GlcNAc + H2O hydrolysis of Man-beta-(1-4)GlcNAc linkage in the trimannosyl core structure of N-glycans Lilium longiflorum ?
-
 ?
3.2.1.152 Man-beta-(1-4)GlcNAc + H2O hydrolysis of Man-beta-(1-4)GlcNAc linkage in the trimannosyl core structure of N-glycans Arabidopsis thaliana ?
-
 ?
3.2.1.152 additional information enzyme does not hydrolyze pyridylamino-sugar chains containing Man-alpha-(1-3)Man-beta or Xylose-beta-(1-2)Man-beta, enzyme does not hydrolyze p-nitrophenyl-beta-mannoside Lilium longiflorum ?
-
 ?
3.2.1.152 additional information enzyme does not hydrolyze pyridylamino-sugar chains containing Man-alpha-(1-3)Man-beta or Xylose-beta-(1-2)Man-beta, enzyme does not hydrolyze p-nitrophenyl-beta-mannoside Arabidopsis thaliana ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.152 ? three bands on SDS-PAGE of 42000, 31000, and 28000 Da Lilium longiflorum

Synonyms

EC Number Synonyms Comment Organism
3.2.1.152 endo-beta-mannosidase
-
 Lilium longiflorum
3.2.1.152 endo-beta-mannosidase
-
 Arabidopsis thaliana

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.152 5
-
-
 Arabidopsis thaliana

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.2.1.152 3 7 unstable below pH 3 and above pH 7 Arabidopsis thaliana