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Literature summary extracted from

  • Hoshi, H.; Nakagawa, H.; Nishiguchi, S.; Iwata, K.; Niikura, K.; Monde, K.; Nishimura, S.
    An engineered hyaluronan synthase: characterization for recombinant human hyaluronan synthase 2 Escherichia coli (2004), J. Biol. Chem., 279, 2341-2349.
    View publication on PubMed

Application

EC Number Application Comment Organism
2.4.1.212 biotechnology optimization of the recombinant enzyme expression in Escherichia coli for large scale production of hyaluronan polymers for usage in basic studies, and for biotechnological creation of functional carbohydrates in medical purposes, engineering of produced product chain length Homo sapiens
2.4.1.212 synthesis optimization of the recombinant enzyme expression in Escherichia coli for large scale production of hyaluronan polymers for usage in basic studies, and for biotechnological creation of functional carbohydrates in medical purposes, engineering of produced product chain length Homo sapiens

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.4.1.212 expression of isozyme HAS2-MBP-fusion protein in Escherichia coli strain JM109 Homo sapiens

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.4.1.212 Mg2+
-
 Homo sapiens

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.4.1.212 UDP-N-acetyl-D-glucosamine + UDP-D-glucuronate Homo sapiens addition of monosaccharides to the linear heteropolysaccharide chain [beta-N-acetyl-D-glucosaminyl(1-4)beta-D-glucuronosyl(1-3)]n + UDP
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.4.1.212 Homo sapiens
-
 isozyme HAS2
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.1.212 UDP-N-acetyl-D-glucosamine + UDP-D-glucuronate addition of monosaccharides to the linear heteropolysaccharide chain Homo sapiens [beta-N-acetyl-D-glucosaminyl(1-4)beta-D-glucuronosyl(1-3)]n + UDP
-
 ?
2.4.1.212 UDP-N-acetyl-D-glucosamine + UDP-D-glucuronate addition of monosaccharides to the linear heteropolysaccharide chain, recombinant isozyme HAS2 prefers the production of a mixture of 8mers and 16mers Homo sapiens [beta-N-acetyl-D-glucosaminyl(1-4)beta-D-glucuronosyl(1-3)]n + UDP
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.4.1.212 HAS
-
 Homo sapiens

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.4.1.212 37
-
 assay at Homo sapiens

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.4.1.212 7.4
-
 assay at Homo sapiens