EC Number | Activating Compound | Comment | Organism | Structure |
---|---|---|---|---|
2.1.1.156 | additional information | abiotic stess-inducible enzyme, e.g. high salt content | Aphanothece halophytica | |
2.1.1.157 | additional information | abiotic stess-inducible enzyme, e.g. high salt content | Aphanothece halophytica |
EC Number | Cloned (Comment) | Organism |
---|---|---|
2.1.1.156 | expressed in Escherichia coli BL21(DE3) | Aphanothece halophytica |
2.1.1.156 | orf1, DNA and amino acid sequence determination and analysis, phylogenetic analysis, overexpression of wild-type and mutant enzymes in Escherichia coli, functional coexpression of the soluble enzyme with the sarcosine dimethylglycine methyltransferase, EC 2.1.1.157, increases the intracellular betaine level and the growth rate of Escherichia coli strain BL21(DE3) | Aphanothece halophytica |
2.1.1.157 | orf2, DNA and amino acid sequence determination and analysis, phylogenetic analysis, overexpression of wild-type and mutant enzymes in Escherichia coli, functional coexpression of the soluble enzyme with the glycine sarcosine methyltransferase, EC 2.1.1.156, increases the intracellular betaine level and the growth rate of Escherichia coli strain BL21(DE3) | Aphanothece halophytica |
2.1.1.161 | expressed in Escherichia coli BL21(DE3) | Aphanothece halophytica |
EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
2.1.1.156 | R169E | inactive | Aphanothece halophytica |
2.1.1.156 | R169E | site-directed mutagenesis, completely inactive mutant | Aphanothece halophytica |
2.1.1.156 | R169K | site-directed mutagenesis, reduced activity and highly increased Km for ATP with both glycine and sarcosine compared to the wild-type enzyme | Aphanothece halophytica |
2.1.1.156 | R169K | drastic increase of Km of the methyl-acceptor substrates, 2-fold increase in Km for S-adenosyl-L-methionine | Aphanothece halophytica |
2.1.1.157 | M172R | site-directed mutagenesis, reduced activity with sarcosine, but highly reduced activity with dimethylglycine compared to the wild-type enzyme | Aphanothece halophytica |
2.1.1.157 | P171Q | site-directed mutagenesis, reduced activity with sarcosine and dimethylglycine compared to the wild-type enzyme | Aphanothece halophytica |
2.1.1.157 | P171Q/M172R | site-directed mutagenesis, reduced activity with glycine and sarcosine, but highly reduced activity with dimethylglycine compared to the wild-type enzyme | Aphanothece halophytica |
2.1.1.161 | M172R | almost inactive | Aphanothece halophytica |
2.1.1.161 | P171Q | strongly increased Km for dimethylglycine, slight increase in the Km for S-adenosyl-L-methionine | Aphanothece halophytica |
2.1.1.161 | P171Q/M172R | almost inactive | Aphanothece halophytica |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
2.1.1.156 | acetate | 80% inhibition at 250 mM, with glycine | Aphanothece halophytica | |
2.1.1.156 | dimethylglycine | versus glycine, 65% inhibition at 250 mM | Aphanothece halophytica | |
2.1.1.156 | ethanolamine | 45% inhibition at 250 mM, with glycine | Aphanothece halophytica | |
2.1.1.156 | KCl | - |
Aphanothece halophytica | |
2.1.1.156 | monomethyl ethanolamine | 35% inhibition at 250 mM, with glycine | Aphanothece halophytica | |
2.1.1.156 | additional information | inhibition rates with sarcosine, overview, no feedback inhibition by betaine up to 2 M, no inhibition with glycine by serine, methionine, asparagine, glutamate, cysteine, and alanine; no inhibition with betaine | Aphanothece halophytica | |
2.1.1.156 | N,N-dimethylglycine | - |
Aphanothece halophytica | |
2.1.1.156 | NaCl | - |
Aphanothece halophytica | |
2.1.1.156 | phenylalanine | 10% inhibition at 250 mM, with glycine | Aphanothece halophytica | |
2.1.1.156 | proline | 30% inhibition at 250 mM, with glycine | Aphanothece halophytica | |
2.1.1.156 | S-adenosyl-L-homocysteine | product inhibition | Aphanothece halophytica | |
2.1.1.156 | sarcosine | competitive inhibition, versus glycine, 70% inhibition at 250 mM | Aphanothece halophytica | |
2.1.1.157 | dimethylglycine | competitive inhibition, versus sarcosine, 40% inhibition at 250 mM | Aphanothece halophytica | |
2.1.1.157 | isovaleric acid | 10% inhibition at 250 mM, with dimethylglycine | Aphanothece halophytica | |
2.1.1.157 | L-alanine | 10% inhibition at 250 mM, with dimethylglycine | Aphanothece halophytica | |
2.1.1.157 | L-cysteine | 10% inhibition at 250 mM, with dimethylglycine | Aphanothece halophytica | |
2.1.1.157 | L-methionine | 10% inhibition at 250 mM, with dimethylglycine | Aphanothece halophytica | |
2.1.1.157 | L-phenylalanine | 10% inhibition at 250 mM | Aphanothece halophytica | |
2.1.1.157 | additional information | inhibition rates with sarcosine, overview, no inhibition with dimethylglycine by glycine, acetate, ethanolamine, monomethylethanolamine, serine, asparagine, glutamate, and proline | Aphanothece halophytica | |
2.1.1.157 | n-butyric acid | 50% inhibition at 250 mM, with dimethylglycine | Aphanothece halophytica | |
2.1.1.157 | propionic acid | 10% inhibition at 250 mM, with dimethylglycine | Aphanothece halophytica | |
2.1.1.157 | S-adenosyl-L-homocysteine | product inhibition | Aphanothece halophytica | |
2.1.1.157 | sarcosine | slight competitive inhibition, versus dimethylglycine, 20% inhibition at 250 mM | Aphanothece halophytica | |
2.1.1.157 | tert-butylacetic acid | 30% inhibition at 250 mM, with dimethylglycine | Aphanothece halophytica | |
2.1.1.161 | alanine | slight inhibition | Aphanothece halophytica | |
2.1.1.161 | cysteine | slight inhibition | Aphanothece halophytica | |
2.1.1.161 | isovaleric acid | slight inhibition | Aphanothece halophytica | |
2.1.1.161 | KCl | - |
Aphanothece halophytica | |
2.1.1.161 | methionine | slight inhibition | Aphanothece halophytica | |
2.1.1.161 | additional information | no inhibition with betaine | Aphanothece halophytica | |
2.1.1.161 | n-butyric acid | - |
Aphanothece halophytica | |
2.1.1.161 | N-methylglycine | slight inhibition | Aphanothece halophytica | |
2.1.1.161 | NaCl | - |
Aphanothece halophytica | |
2.1.1.161 | phenylalanine | slight inhibition | Aphanothece halophytica | |
2.1.1.161 | propionic acid | slight inhibition | Aphanothece halophytica | |
2.1.1.161 | S-adenosyl-L-homocysteine | - |
Aphanothece halophytica | |
2.1.1.161 | t-butyrylacetic acid | - |
Aphanothece halophytica |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.1.1.156 | 0.6 | - |
S-adenosyl-L-methionine | pH 8.8, 37°C, recombinant wild-type enzyme, with sarcosine | Aphanothece halophytica | |
2.1.1.156 | 0.6 | - |
S-adenosyl-L-methionine | with N-methylglycine as methyl group acceptor, pH 8.8, 37°C | Aphanothece halophytica | |
2.1.1.156 | 0.7 | - |
S-adenosyl-L-methionine | pH 8.8, 37°C, recombinant wild-type enzyme, with glycine | Aphanothece halophytica | |
2.1.1.156 | 0.7 | - |
S-adenosyl-L-methionine | with glycine as methyl group acceptor, pH 8.8, 37°C | Aphanothece halophytica | |
2.1.1.156 | 0.8 | - |
N-methylglycine | pH 8.8, 37°C | Aphanothece halophytica | |
2.1.1.156 | 0.8 | - |
sarcosine | pH 8.8, 37°C, recombinant wild-type enzyme | Aphanothece halophytica | |
2.1.1.156 | 1 | - |
glycine | pH 8.8, 37°C, recombinant mutant R169K | Aphanothece halophytica | |
2.1.1.156 | 1.1 | - |
sarcosine | pH 8.8, 37°C, recombinant mutant R169K | Aphanothece halophytica | |
2.1.1.156 | 1.5 | - |
glycine | pH 8.8, 37°C | Aphanothece halophytica | |
2.1.1.156 | 1.5 | - |
glycine | pH 8.8, 37°C, recombinant wild-type enzyme | Aphanothece halophytica | |
2.1.1.156 | 50 | - |
S-adenosyl-L-methionine | pH 8.8, 37°C, recombinant mutant R169K, with sarcosine | Aphanothece halophytica | |
2.1.1.156 | 75 | - |
S-adenosyl-L-methionine | pH 8.8, 37°C, recombinant mutant R169K, with glycine | Aphanothece halophytica | |
2.1.1.157 | 0.18 | - |
S-adenosyl-L-methionine | pH 8.8, 37°C, recombinant wild-type enzyme, with dimethylglycine | Aphanothece halophytica | |
2.1.1.157 | 0.25 | - |
S-adenosyl-L-methionine | pH 8.8, 37°C, recombinant mutant P171Q, with dimethylglycine | Aphanothece halophytica | |
2.1.1.157 | 0.5 | - |
dimethylglycine | pH 8.8, 37°C, recombinant wild-type enzyme | Aphanothece halophytica | |
2.1.1.157 | 0.6 | - |
S-adenosyl-L-methionine | pH 8.8, 37°C, recombinant wild-type enzyme, with sarcosine | Aphanothece halophytica | |
2.1.1.157 | 0.8 | - |
sarcosine | pH 8.8, 37°C, recombinant wild-type enzyme | Aphanothece halophytica | |
2.1.1.157 | 7.5 | - |
dimethylglycine | pH 8.8, 37°C, recombinant mutant P171Q | Aphanothece halophytica | |
2.1.1.161 | 0.18 | - |
S-adenosyl-L-methionine | pH 8.8, 37°C | Aphanothece halophytica | |
2.1.1.161 | 0.5 | - |
N,N-dimethylglycine | pH 8.8, 37°C | Aphanothece halophytica |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
2.1.1.156 | 31210 | - |
calculated from the deduced amino acid sequence | Aphanothece halophytica |
2.1.1.156 | 32000 | - |
gel filtration | Aphanothece halophytica |
2.1.1.156 | 32000 | - |
recombinant enzyme, gel filtration | Aphanothece halophytica |
2.1.1.156 | 33000 | - |
1 * 33000, recombinant enzyme, SDS-PAGE | Aphanothece halophytica |
2.1.1.156 | 33000 | - |
1 * 33000, SDS-PAGE, 1 * 32000, gel filtration | Aphanothece halophytica |
2.1.1.157 | 29000 | - |
recombinant enzyme, gel filtration | Aphanothece halophytica |
2.1.1.157 | 31000 | - |
1 * 31000, recombinant enzyme, SDS-PAGE | Aphanothece halophytica |
2.1.1.161 | 29000 | - |
gel filtration | Aphanothece halophytica |
2.1.1.161 | 31000 | - |
1 * 31000, SDS-PAGE, 1 * 29000, gel filtration | Aphanothece halophytica |
2.1.1.161 | 31210 | - |
calculated from the deduced amino acid sequence | Aphanothece halophytica |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.1.1.156 | additional information | Aphanothece halophytica | the enzyme is involved in the alternate biosynthesis of betaine, which is an important osmoprotectant and is synthesized in response to abiotic stress | ? | - |
? | |
2.1.1.156 | S-adenosyl-L-methionine + glycine | Aphanothece halophytica | - |
S-adenosyl-L-homocysteine + sarcosine | - |
? | |
2.1.1.156 | S-adenosyl-L-methionine + glycine | Aphanothece halophytica | synthesis of the compatible solute betaine de novo through the methylation of glycine, sarcosine and dimethylglycine with the methyl group from S-adenosylmethionine | S-adenosyl-L-homocysteine + N-methylglycine | - |
? | |
2.1.1.156 | S-adenosyl-L-methionine + N-methylglycine | Aphanothece halophytica | synthesis of the compatible solute betaine de novo through the methylation of glycine, sarcosine and dimethylglycine with the methyl group from S-adenosylmethionine | S-adenosyl-L-homocysteine + N,N-dimethylglycine | - |
? | |
2.1.1.156 | S-adenosyl-L-methionine + sarcosine | Aphanothece halophytica | - |
S-adenosyl-L-homocysteine + N,N-dimethylglycine | - |
? | |
2.1.1.157 | additional information | Aphanothece halophytica | the enzyme is involved in the alternate biosynthesis of betaine, which is an important osmoprotectant and is synthesized in response to abiotic stress | ? | - |
? | |
2.1.1.157 | S-adenosyl-L-methionine + N,N-dimethylglycine | Aphanothece halophytica | - |
S-adenosyl-L-homocysteine + betaine | i.e. N,N,N-trimethylglycine | ? | |
2.1.1.157 | S-adenosyl-L-methionine + sarcosine | Aphanothece halophytica | - |
S-adenosyl-L-homocysteine + N,N-dimethylglycine | - |
? | |
2.1.1.161 | S-adenosyl-L-methionine + N,N-dimethylglycine | Aphanothece halophytica | synthesis of the compatible solute betaine de novo through the methylation of glycine, sarcosine and dimethylglycine with the methyl group from S-adenosylmethionine | S-adenosyl-L-homocysteine + N,N,N-trimethylglycine | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
2.1.1.156 | Aphanothece halophytica | - |
- |
- |
2.1.1.156 | Aphanothece halophytica | Q83WC4 | inducible enzyme | - |
2.1.1.157 | Aphanothece halophytica | Q83WC3 | inducible enzyme | - |
2.1.1.161 | Aphanothece halophytica | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
2.1.1.156 | - |
Aphanothece halophytica |
2.1.1.156 | recombinant enzyme from Escherichia coli by ammonium sulfate fractionation and ion exchange chromatography to homogeneity | Aphanothece halophytica |
2.1.1.157 | recombinant enzyme from Escherichia coli by ammonium sulfate fractionation and ion exchange chromatography to homogeneity | Aphanothece halophytica |
2.1.1.161 | - |
Aphanothece halophytica |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.1.1.156 | additional information | the enzyme is involved in the alternate biosynthesis of betaine, which is an important osmoprotectant and is synthesized in response to abiotic stress | Aphanothece halophytica | ? | - |
? | |
2.1.1.156 | additional information | enzyme shows strict substrate specificity | Aphanothece halophytica | ? | - |
? | |
2.1.1.156 | S-adenosyl-L-methionine + glycine | - |
Aphanothece halophytica | S-adenosyl-L-homocysteine + sarcosine | - |
? | |
2.1.1.156 | S-adenosyl-L-methionine + glycine | - |
Aphanothece halophytica | S-adenosyl-L-homocysteine + sarcosine | i.e. 2-methylglycine | ? | |
2.1.1.156 | S-adenosyl-L-methionine + glycine | - |
Aphanothece halophytica | S-adenosyl-L-homocysteine + N-methylglycine | - |
? | |
2.1.1.156 | S-adenosyl-L-methionine + glycine | synthesis of the compatible solute betaine de novo through the methylation of glycine, sarcosine and dimethylglycine with the methyl group from S-adenosylmethionine | Aphanothece halophytica | S-adenosyl-L-homocysteine + N-methylglycine | - |
? | |
2.1.1.156 | S-adenosyl-L-methionine + N-methylglycine | - |
Aphanothece halophytica | S-adenosyl-L-homocysteine + N,N-dimethylglycine | - |
? | |
2.1.1.156 | S-adenosyl-L-methionine + N-methylglycine | synthesis of the compatible solute betaine de novo through the methylation of glycine, sarcosine and dimethylglycine with the methyl group from S-adenosylmethionine | Aphanothece halophytica | S-adenosyl-L-homocysteine + N,N-dimethylglycine | - |
? | |
2.1.1.156 | S-adenosyl-L-methionine + sarcosine | - |
Aphanothece halophytica | S-adenosyl-L-homocysteine + N,N-dimethylglycine | - |
? | |
2.1.1.157 | additional information | the enzyme is involved in the alternate biosynthesis of betaine, which is an important osmoprotectant and is synthesized in response to abiotic stress | Aphanothece halophytica | ? | - |
? | |
2.1.1.157 | additional information | enzyme shows strict substrate specificity | Aphanothece halophytica | ? | - |
? | |
2.1.1.157 | S-adenosyl-L-methionine + glycine | low activity | Aphanothece halophytica | S-adenosyl-L-homocysteine + sarcosine | - |
? | |
2.1.1.157 | S-adenosyl-L-methionine + N,N-dimethylglycine | - |
Aphanothece halophytica | S-adenosyl-L-homocysteine + betaine | i.e. N,N,N-trimethylglycine | ? | |
2.1.1.157 | S-adenosyl-L-methionine + sarcosine | - |
Aphanothece halophytica | S-adenosyl-L-homocysteine + N,N-dimethylglycine | - |
? | |
2.1.1.157 | S-adenosyl-L-methionine + sarcosine | i.e. methylglycine | Aphanothece halophytica | S-adenosyl-L-homocysteine + N,N-dimethylglycine | - |
? | |
2.1.1.161 | S-adenosyl-L-methionine + N,N-dimethylglycine | - |
Aphanothece halophytica | S-adenosyl-L-homocysteine + N,N,N-trimethylglycine | - |
? | |
2.1.1.161 | S-adenosyl-L-methionine + N,N-dimethylglycine | synthesis of the compatible solute betaine de novo through the methylation of glycine, sarcosine and dimethylglycine with the methyl group from S-adenosylmethionine | Aphanothece halophytica | S-adenosyl-L-homocysteine + N,N,N-trimethylglycine | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
2.1.1.156 | monomer | 1 * 33000, recombinant enzyme, SDS-PAGE | Aphanothece halophytica |
2.1.1.156 | monomer | 1 * 33000, SDS-PAGE, 1 * 32000, gel filtration | Aphanothece halophytica |
2.1.1.156 | More | enzyme aggregated to tetramers is inactive | Aphanothece halophytica |
2.1.1.157 | monomer | 1 * 31000, recombinant enzyme, SDS-PAGE | Aphanothece halophytica |
2.1.1.161 | monomer | 1 * 31000, SDS-PAGE, 1 * 29000, gel filtration | Aphanothece halophytica |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
2.1.1.156 | GSMT | - |
Aphanothece halophytica |
2.1.1.157 | SDMT | - |
Aphanothece halophytica |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
2.1.1.156 | 37 | - |
assay at | Aphanothece halophytica |
2.1.1.157 | 37 | - |
assay at | Aphanothece halophytica |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
2.1.1.156 | 8.8 | - |
assay at | Aphanothece halophytica |
2.1.1.156 | 9 | - |
glycine or N-methylglycine | Aphanothece halophytica |
2.1.1.157 | 8.8 | - |
assay at | Aphanothece halophytica |
2.1.1.161 | 9 | - |
- |
Aphanothece halophytica |
EC Number | pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|---|
2.1.1.156 | 8 | 11 | glycine or N-methylglycine, very low activity below pH 8 | Aphanothece halophytica |
2.1.1.161 | 7 | 11 | very low activity below pH 7 | Aphanothece halophytica |