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Literature summary extracted from

  • Wu, J.; Howe, D.L.; Woodard, R.W.
    Thermotoga maritima 3-deoxy-D-arabino-heptulosonate 7-phosphate (DAHP) synthase: the ancestral eubacterial DAHP synthase? (2003), J. Biol. Chem., 278, 27525-27531.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.5.1.54
-
 Thermotoga maritima

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.5.1.54 EDTA inactivation, reactivation in decreasing order by Zn2+, Cd2+, Mn2+, Cu2+, Co2+, Ni2+ Thermotoga maritima
2.5.1.54 L-phenylalanine 1 mM, 32% residual activity Thermotoga maritima
2.5.1.54 L-tyrosine 1 mM, 23% residual activity Thermotoga maritima
2.5.1.54 additional information not inhibitory: L-tryptophan, chorismate, shikimate, D-phenylalanine, L-histidine Thermotoga maritima

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.5.1.54 0.0095
-
 phosphoenolpyruvate pH 7.5, 70°C Thermotoga maritima
2.5.1.54 0.0117
-
 phosphoenolpyruvate pH 7.5, 60°C Thermotoga maritima
2.5.1.54 0.013
-
 phosphoenolpyruvate pH 7.5, 50°C Thermotoga maritima
2.5.1.54 0.0573
-
 D-erythrose 4-phosphate pH 7.5, 50°C Thermotoga maritima
2.5.1.54 0.138
-
 D-erythrose 4-phosphate pH 7.5, 60°C Thermotoga maritima
2.5.1.54 0.3506
-
 D-erythrose 4-phosphate pH 7.5, 70°C Thermotoga maritima

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.5.1.54 additional information reactivation of EDTA-inactivated enzyme in decreasing order by Zn2+, Cd2+, Mn2+, Cu2+, Co2+, Ni2+ Thermotoga maritima
2.5.1.54 Zn2+
-
 Thermotoga maritima

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.5.1.54 38000
-
 4 * 38000, SDS-PAGE Thermotoga maritima
2.5.1.54 134000
-
 gel filtration Thermotoga maritima

Organism

EC Number Organism UniProt Comment Textmining
2.5.1.54 Thermotoga maritima
-
 recombinant enzyme
-

Renatured (Commentary)

EC Number Renatured (Comment) Organism
2.5.1.54 reactivation of EDTA-inactivated enzyme in decreasing order by Zn2+, Cd2+, Mn2+, Cu2+, Co2+, Ni2+ Thermotoga maritima

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.5.1.54 phosphoenolpyruvate + D-erythrose 4-phosphate + H2O
-
 Thermotoga maritima 3-deoxy-D-erythro-hept-2-ulosonate 7-phosphate + phosphate
-
 ?

Subunits

EC Number Subunits Comment Organism
2.5.1.54 tetramer 4 * 38000, SDS-PAGE Thermotoga maritima

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.5.1.54 90
-
-
 Thermotoga maritima

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.5.1.54 60
-
 86h, 50% residual activity Thermotoga maritima
2.5.1.54 70
-
 21h, 50% residual activity Thermotoga maritima
2.5.1.54 80
-
 5h, 50% residual activity Thermotoga maritima

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.5.1.54 2.3
-
 phosphoenolpyruvate pH 7.5, 50°C Thermotoga maritima
2.5.1.54 5
-
 phosphoenolpyruvate pH 7.5, 60°C Thermotoga maritima
2.5.1.54 7.6
-
 phosphoenolpyruvate pH 7.5, 70°C Thermotoga maritima

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.5.1.54 6.3
-
 60°C Thermotoga maritima

pH Range

EC Number pH Minimum pH Maximum Comment Organism
2.5.1.54 6 7 more than 90% of maximum activity Thermotoga maritima