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Literature summary extracted from

  • Choi, K.B.; Morishige, T.; Shitan, N.; Yazaki, K.; Sato, F.
    Molecular cloning and characterization of coclaurine N-methyltransferase from cultured cells of Coptis japonica (2002), J. Biol. Chem., 277, 830-835.
    View publication on PubMed

Application

EC Number Application Comment Organism
2.1.1.140 biotechnology CNMT should be quite useful for biotransformation of the intermediates of alkaloid biosynthesis to N-methyltransferred products Coptis japonica

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.1.1.140 expression in Escherichia coli Coptis japonica

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.1.1.140 Co2+ 5 mM, 37% inhibition Coptis japonica
2.1.1.140 Cu2+ 5 mM, 52% inhibition Coptis japonica
2.1.1.140 Mn2+ 5 mM, 52% inhibition Coptis japonica

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.1.1.140 0.16
-
 (R,S)-norreticuline recombinant CNMT Coptis japonica
2.1.1.140 0.39
-
 S-adenosyl-L-methionine recombinant CNMT Coptis japonica

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.1.1.140 S-adenosyl-L-methionine + (S)-coclaurine Coptis japonica isoquinoline alkaloid biosynthesis S-adenosyl-L-homocysteine + (S)-N-methylcoclaurine
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.1.1.140 Coptis japonica
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.1.1.140 recombinant enzyme Coptis japonica

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.1.1.140 cell culture
-
 Coptis japonica
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.1.1.140 1.08
-
-
 Coptis japonica

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.1.1.140 additional information no activity with: (R,S)-scoulerine, 1,2,3,4-tetrahydroisoquinoline and 1,2,3,4-tetrahydro-3-isoquinoline carboxylic acid (+)-emetine Coptis japonica ?
-
 ?
2.1.1.140 S-adenosyl-L-methionine + (R)-coclaurine 240% of the activity with (R,S)-norreticuline Coptis japonica S-adenosyl-L-homocysteine + (R)-N-methylcoclaurine
-
 ?
2.1.1.140 S-adenosyl-L-methionine + (R,S)-6-O-methylnorlaudanosoline 69% of the activity with (R,S)-norreticuline Coptis japonica S-adenosyl-L-homocysteine + (R,S)-N-methyl-6-O-methylnorlaudanosoline
-
 ?
2.1.1.140 S-adenosyl-L-methionine + (R,S)-norlaudanosoline 49% of the activity with (R,S)-norreticuline Coptis japonica S-adenosyl-L-homocysteine + (R,S)-N-methylnorlaudanosoline
-
 ?
2.1.1.140 S-adenosyl-L-methionine + (S)-coclaurine isoquinoline alkaloid biosynthesis Coptis japonica S-adenosyl-L-homocysteine + (S)-N-methylcoclaurine
-
 ?
2.1.1.140 S-adenosyl-L-methionine + (S)-coclaurine sequential mechanism, 153% of the activity with (R,S)-norreticuline Coptis japonica S-adenosyl-L-homocysteine + (S)-N-methylcoclaurine
-
 ?
2.1.1.140 S-adenosyl-L-methionine + 1-methyl-6,7-dihydroxy-1,2,3,4-tetrahydroisoquinoline 15% of the activity with (R,S)-norreticuline Coptis japonica S-adenosyl-L-homocysteine + 6,7-dihydroxy-1,N-dimethyl-1,2,3,4-tetrahydroisoquinoline
-
 ?
2.1.1.140 S-adenosyl-L-methionine + 6,7-dimethoxy-N-methyl-1,2,3,4-tetrahydroisoquinoline 180% of the activity with (R,S)-norreticuline Coptis japonica S-adenosyl-L-homocysteine + 6,7-dimethoxy-1,2,3,4-tetrahydroisoquinoline
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.1.1.140 CNMT
-
 Coptis japonica
2.1.1.140 coclaurine N-methyltransferase
-
 Coptis japonica
2.1.1.140 S-adenosyl-L-methionine:coclaurine N-methyltransferase
-
 Coptis japonica

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.1.1.140 7
-
-
 Coptis japonica

pH Range

EC Number pH Minimum pH Maximum Comment Organism
2.1.1.140 6.5 8 about 50% of maximal activity at pH 6.5 and at pH 8.0 Coptis japonica