EC Number | Activating Compound | Comment | Organism | Structure |
---|---|---|---|---|
1.8.1.4 | Zn2+ | stimulates the oxygen oxidase activity of the enzyme | Sus scrofa |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
1.8.1.4 | NAD+ | product inhibition of the oxygen oxidase activity | Sus scrofa | |
1.8.1.4 | Zn2+ | reversible binding, competitive to lipoamide, uncompetitive to NADH | Sus scrofa |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.8.1.4 | additional information | - |
additional information | dehydrogenase and oxidase activity kinetics | Sus scrofa |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.8.1.4 | Sus scrofa | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
1.8.1.4 | further purification of the commercial preparation by gel filtration | Sus scrofa |
EC Number | Reaction | Comment | Organism | Reaction ID |
---|---|---|---|---|
1.8.1.4 | protein N6-(dihydrolipoyl)lysine + NAD+ = protein N6-(lipoyl)lysine + NADH + H+ | ping pong mechanism | Sus scrofa |
EC Number | Source Tissue | Comment | Organism | Textmining |
---|---|---|---|---|
1.8.1.4 | commercial preparation | - |
Sus scrofa | - |
1.8.1.4 | heart | - |
Sus scrofa | - |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.8.1.4 | DL-6,8-thiooctic acid amide + NADH | - |
Sus scrofa | ? + NAD+ | - |
r | |
1.8.1.4 | lipoamide + NADH | - |
Sus scrofa | dihydrolipoamide + NAD+ | - |
r | |
1.8.1.4 | O2 + NADH | the enzyme produces reactive oxygen species, i.e. hydrogen peroxide, acting as an oxidase | Sus scrofa | H2O2 + NAD+ | - |
? |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
1.8.1.4 | LADH | - |
Sus scrofa |
1.8.1.4 | lipoamide dehydrogenase | - |
Sus scrofa |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
1.8.1.4 | 20 | - |
assay at | Sus scrofa |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
1.8.1.4 | 7.5 | - |
assay at | Sus scrofa |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
1.8.1.4 | FAD | - |
Sus scrofa | |
1.8.1.4 | NAD+ | - |
Sus scrofa | |
1.8.1.4 | NADH | - |
Sus scrofa |
EC Number | Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.8.1.4 | additional information | - |
additional information | Zn2+ inhibition kinetics and mechanism | Sus scrofa |