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Literature summary extracted from

  • Gijsbers, R.; Ceulemans, H.; Stalmans, W.; Bollen, M.
    Structural and catalytic similarities between nucleotide pyrophosphatases/phosphodiesterases and alkaline phosphatases (2001), J. Biol. Chem., 276, 1361-1368.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.6.1.9 mutants expressed in COS-1 cells Mus musculus

Protein Variants

EC Number Protein Variants Comment Organism
3.6.1.9 D200N mutation abolishes formation of the nucleotidylated intermediate Mus musculus
3.6.1.9 D358N mutant enzyme can still form nucleotidylated catalytic intermediates but is hampered in the second step of catalysis Mus musculus
3.6.1.9 F239A mutation halves activity, decreased ability to trap the catalytic intermediate Mus musculus
3.6.1.9 F239Q decreased activity and level of covalent intermediate of the mutant may be accounted for by a decreased binding of the substrate Mus musculus
3.6.1.9 H362Q mutant enzyme can still form nucleotidylated catalytic intermediates but is hampered in the second step of catalysis Mus musculus
3.6.1.9 H406Q mutation abolishes formation of the nucleotidylated intermediate Mus musculus
3.6.1.9 K237A mutation halves activity Mus musculus
3.6.1.9 T238S mutant enzyme can still form nucleotidylated catalytic intermediates but is hampered in the second step of catalysis Mus musculus

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.6.1.9 EDTA activity can be restored by addition of 2-5 mM ZnCl2 or CaCl2, but only partially by 2-5 mM MgCl2 Mus musculus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.6.1.9 CaCl2 2-5 mM restores activity of EDTA-inhibited enzyme Mus musculus
3.6.1.9 MgCl2 2-5 mM partially restores activity of EDTA-inhibited enzyme Mus musculus
3.6.1.9 ZnCl2 2-5 mM restores activity of EDTA-inhibited enzyme Mus musculus

Organism

EC Number Organism UniProt Comment Textmining
3.6.1.9 Mus musculus Q9EQG7
-
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
3.6.1.9 phosphoprotein mutations D358N and H362Q still allow a weak labeling, whereas mutation His517, Asp200, Asp405 and His406 completely abolish autophosphorylation in presence of ATP Mus musculus

Synonyms

EC Number Synonyms Comment Organism
3.6.1.9 NPP1
-
 Mus musculus
3.6.1.9 nucleotide pyrophosphatase
-
 Mus musculus