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Literature summary extracted from

  • Kim, H.
    Asparagine-473 residue is important to the efficient function of human dihydrolipoamide dehydrogenase (2005), J. Biochem. Mol. Biol., 38, 248-252.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.8.1.4 expression of D473L mutant in Escherichia coli strain XL1-Blue Homo sapiens

Protein Variants

EC Number Protein Variants Comment Organism
1.8.1.4 D473L site-directed mutagenesis, mutant shows about 37fold decreased activity and small conformational changes compared to the wild-type enzyme Homo sapiens

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.8.1.4 dihydrolipoamide + NAD+ Homo sapiens
-
 lipoamide + NADH
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.8.1.4 Homo sapiens
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.8.1.4 recombinant D473L mutant from Escherichia coli to homogeneity Homo sapiens

Reaction

EC Number Reaction Comment Organism Reaction ID
1.8.1.4 protein N6-(dihydrolipoyl)lysine + NAD+ = protein N6-(lipoyl)lysine + NADH + H+ Asp473 is important for efficient catalytic function of the enzyme Homo sapiens
a

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.8.1.4 16.6
-
 purified recombinant mutant D473L Homo sapiens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.8.1.4 dihydrolipoamide + NAD+
-
 Homo sapiens lipoamide + NADH
-
 ?

Subunits

EC Number Subunits Comment Organism
1.8.1.4 More the homodimeric enzyme is the E3 component of 2-ketoacid dehydrogenase multienzyme complex Homo sapiens

Synonyms

EC Number Synonyms Comment Organism
1.8.1.4 dihydrolipoamide dehydrogenase
-
 Homo sapiens
1.8.1.4 dihydrolipoamide:NAD+ oxidoreductase
-
 Homo sapiens
1.8.1.4 E3
-
 Homo sapiens
1.8.1.4 More enzyme is the E3 component of 2-ketoacid dehydrogenase multienzyme complex Homo sapiens

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.8.1.4 37
-
 assay at Homo sapiens

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.8.1.4 8
-
 assay at Homo sapiens

Cofactor

EC Number Cofactor Comment Organism Structure
1.8.1.4 FAD prosthetic group, 1 FAD per enzyme subunit Homo sapiens
1.8.1.4 NAD+
-
 Homo sapiens