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Literature summary extracted from

  • Yamaguchi, K.; Kawamura, A.; Ogawa, H.; Suzuki, S.
    Characterization of nitrous oxide reductase from a methylotrophic denitrifying bacterium, Hyphomicrobium denitrificans A3151 (2003), J. Biochem., 134, 853-858.
    View publication on PubMed

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.7.2.4 copper form A and B contain 9.0 and 8.2 Cu atoms per dimer, respectively Hyphomicrobium denitrificans

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.7.2.4 65000
-
 2 * 65000, SDS-PAGE Hyphomicrobium denitrificans
1.7.2.4 105000
-
 gel filtration Hyphomicrobium denitrificans

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.7.2.4 N2O + reduced acceptor Hyphomicrobium denitrificans
-
 N2 + H2O + acceptor
-
 ?
1.7.2.4 N2O + reduced acceptor Hyphomicrobium denitrificans A3151
-
 N2 + H2O + acceptor
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.7.2.4 Hyphomicrobium denitrificans
-
 A3151
-
1.7.2.4 Hyphomicrobium denitrificans A3151
-
 A3151
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.7.2.4 ammonium sulfate, DEAE-Sephadex A-50, Sephacryl S-200 Hyphomicrobium denitrificans

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.7.2.4 29
-
 HdN2OR form A, i.e. reduced form Hyphomicrobium denitrificans
1.7.2.4 45
-
 HdN2OR form A, i.e. oxidized form Hyphomicrobium denitrificans

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.7.2.4 N2O + reduced acceptor
-
 Hyphomicrobium denitrificans N2 + H2O + acceptor
-
 ?
1.7.2.4 N2O + reduced acceptor
-
 Hyphomicrobium denitrificans A3151 N2 + H2O + acceptor
-
 ?
1.7.2.4 N2O + reduced benzyl viologen
-
 Hyphomicrobium denitrificans N2 + H2O + benzyl viologen
-
 ?
1.7.2.4 N2O + reduced benzyl viologen
-
 Hyphomicrobium denitrificans A3151 N2 + H2O + benzyl viologen
-
 ?

Subunits

EC Number Subunits Comment Organism
1.7.2.4 dimer 2 * 65000, SDS-PAGE Hyphomicrobium denitrificans

Synonyms

EC Number Synonyms Comment Organism
1.7.2.4 HdN2OR
-
 Hyphomicrobium denitrificans

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.7.2.4 8.8
-
-
 Hyphomicrobium denitrificans

pH Range

EC Number pH Minimum pH Maximum Comment Organism
1.7.2.4 7 9.5 approx. 50% of maximal activity at pH 7.5, approx. 75% of maximal activity at pH 9.5 Hyphomicrobium denitrificans