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Literature summary extracted from

  • Maillard, A.P.; Biarrotte-Sorin, S.; Villet, R.; Mesnage, S.; Bouhss, A.; Sougakoff, W.; Mayer, C.; Arthur, M.
    Structure-based site-directed mutagenesis of the UDP-MurNAc-pentapeptide-binding cavity of the FemX alanyl transferase from Weissella viridescens (2005), J. Bacteriol., 187, 3833-3838.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.3.2.10 wild type and mutant enzymes expressed as His-tag fusion proteins in Escherichia coli TOP10 Weissella viridescens

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
2.3.2.10 crystallized in the presence of UDP-N-acetylmuramoyl-L-alanyl-D-glutamyl-L-lysyl-D-alanyl-D-alanine by vapor diffusion method Weissella viridescens

Protein Variants

EC Number Protein Variants Comment Organism
2.3.2.10 F70L 35% loss of activity Weissella viridescens
2.3.2.10 K36M activity complete abolished Weissella viridescens
2.3.2.10 K36R 160-fold reduction of activity, 5.4-fold increased Km for UDP-N-acetylmuramoyl-L-alanyl-D-glutamyl-L-lysyl-D-alanyl-D-alanine Weissella viridescens
2.3.2.10 R211K 17000-fold reduction of activity, no effect on Km for Ala-tRNA, Km for UDP-N-acetylmuramoyl-L-alanyl-D-glutamyl-L-lysyl-D-alanyl-D-alanine higher than the highest sample concentration Weissella viridescens
2.3.2.10 Y103F no effect on activity Weissella viridescens
2.3.2.10 Y215F 25-fold decrease of activity, increased Km for UDP-N-acetylmuramoyl-L-alanyl-D-glutamyl-L-lysyl-D-alanyl-D-alanine Weissella viridescens
2.3.2.10 Y215L insoluble protein Weissella viridescens
2.3.2.10 Y256F 2.6-fold reduction of activity Weissella viridescens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.3.2.10 0.0017
-
 L-alanyl-tRNA pH 7.5, 30°C Weissella viridescens
2.3.2.10 0.079
-
 UDP-N-acetylmuramoyl-L-alanyl-D-glutamyl-L-lysyl-D-alanyl-D-alanine pH 7.5, 30°C Weissella viridescens

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.3.2.10 L-alanyl-tRNAAla + UDP-N-acetylmuramoyl-L-alanyl-D-glutamyl-L-lysyl-D-alanyl-D-alanine Weissella viridescens production of precursors for branched chain peptidoglycans tRNAAla + UDP-N-acetylmuramoyl-L-alanyl-D-glutamyl-N6-(L-alanyl)-L-lysyl-D-alanyl-D-alanine
-
 ?
2.3.2.10 L-alanyl-tRNAAla + UDP-N-acetylmuramoyl-L-alanyl-D-glutamyl-L-lysyl-D-alanyl-D-alanine Weissella viridescens CIP 102810 / ATCC 12706 production of precursors for branched chain peptidoglycans tRNAAla + UDP-N-acetylmuramoyl-L-alanyl-D-glutamyl-N6-(L-alanyl)-L-lysyl-D-alanyl-D-alanine
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.3.2.10 Weissella viridescens
-
 formerly Lactobacillus viridescens
-
2.3.2.10 Weissella viridescens CIP 102810 / ATCC 12706
-
 formerly Lactobacillus viridescens
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.3.2.10 recombinant proteins using His-tag Weissella viridescens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.2.10 L-alanyl-tRNAAla + UDP-N-acetylmuramoyl-L-alanyl-D-glutamyl-L-lysyl-D-alanyl-D-alanine
-
 Weissella viridescens tRNAAla + UDP-N-acetylmuramoyl-L-alanyl-D-glutamyl-N6-(L-alanyl)-L-lysyl-D-alanyl-D-alanine
-
 ?
2.3.2.10 L-alanyl-tRNAAla + UDP-N-acetylmuramoyl-L-alanyl-D-glutamyl-L-lysyl-D-alanyl-D-alanine production of precursors for branched chain peptidoglycans Weissella viridescens tRNAAla + UDP-N-acetylmuramoyl-L-alanyl-D-glutamyl-N6-(L-alanyl)-L-lysyl-D-alanyl-D-alanine
-
 ?
2.3.2.10 L-alanyl-tRNAAla + UDP-N-acetylmuramoyl-L-alanyl-D-glutamyl-L-lysyl-D-alanyl-D-alanine
-
 Weissella viridescens CIP 102810 / ATCC 12706 tRNAAla + UDP-N-acetylmuramoyl-L-alanyl-D-glutamyl-N6-(L-alanyl)-L-lysyl-D-alanyl-D-alanine
-
 ?
2.3.2.10 L-alanyl-tRNAAla + UDP-N-acetylmuramoyl-L-alanyl-D-glutamyl-L-lysyl-D-alanyl-D-alanine production of precursors for branched chain peptidoglycans Weissella viridescens CIP 102810 / ATCC 12706 tRNAAla + UDP-N-acetylmuramoyl-L-alanyl-D-glutamyl-N6-(L-alanyl)-L-lysyl-D-alanyl-D-alanine
-
 ?
2.3.2.10 additional information enzyme highly specific for UDP-N-acetylmuramoyl-L-alanyl-D-glutamyl-L-lysyl-D-alanyl-D-alanine, only traces of activity with UDP-N-acetylmuramoyl-tripeptide or UDP-N-acetylmuramoyl-tetrapeptide or derivatives of UDP-N-acetylmuramoyl-pentapeptide Weissella viridescens ?
-
 ?
2.3.2.10 additional information enzyme highly specific for UDP-N-acetylmuramoyl-L-alanyl-D-glutamyl-L-lysyl-D-alanyl-D-alanine, only traces of activity with UDP-N-acetylmuramoyl-tripeptide or UDP-N-acetylmuramoyl-tetrapeptide or derivatives of UDP-N-acetylmuramoyl-pentapeptide Weissella viridescens CIP 102810 / ATCC 12706 ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.3.2.10 FemX
-
 Weissella viridescens

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.3.2.10 26.7
-
 L-alanyl-tRNA pH 7.5, 30°C Weissella viridescens