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Literature summary extracted from

  • Hickman, J.W.; Barber, R.D.; Skaar, E.P.; Donohue, T.J.
    Link between the membrane-bound pyridine nucleotide transhydrogenase and glutathione-dependent processes in Rhodobacter sphaeroides (2002), J. Bacteriol., 184, 400-409.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.6.1.2
-
 Cereibacter sphaeroides

Protein Variants

EC Number Protein Variants Comment Organism
1.6.1.2 additional information enzyme deficient mutants created by insertions at condon 474, 128, and 285 of ptnA Cereibacter sphaeroides

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.6.1.2 membrane
-
 Cereibacter sphaeroides 16020
-

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.6.1.2 NADH + NADP+ Cereibacter sphaeroides links hydride transfer between NAD(H) and NADP(H) to the translocation of protons across membrane, major source of NADPH, provides reducing agent for glutathione and is therefore important for the oxidative stress defense NAD+ + NADPH
-
 r

Organism

EC Number Organism UniProt Comment Textmining
1.6.1.2 Cereibacter sphaeroides
-
 native and enzyme deficient mutant, mutant strain accumulates higher levels of glutathione disulfide
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.6.1.2 additional information
-
 no activity detected with the insertion mutants Cereibacter sphaeroides
1.6.1.2 50.8
-
 native enzyme with acetylpyridine-NAD+ a n NADPH as substrates, pH 7.0 Cereibacter sphaeroides

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.6.1.2 NADH + NADP+
-
 Cereibacter sphaeroides NAD+ + NADPH
-
 r
1.6.1.2 NADH + NADP+ links hydride transfer between NAD(H) and NADP(H) to the translocation of protons across membrane, major source of NADPH, provides reducing agent for glutathione and is therefore important for the oxidative stress defense Cereibacter sphaeroides NAD+ + NADPH
-
 r
1.6.1.2 NADPH + oxidized 3-acetylpyridine adenine dinucleotide
-
 Cereibacter sphaeroides NADP+ + reduced 3-acetylpyridine adenine dinucleotide
-
 ?

Cofactor

EC Number Cofactor Comment Organism Structure
1.6.1.2 NAD(P)+
-
 Cereibacter sphaeroides
1.6.1.2 NAD(P)H
-
 Cereibacter sphaeroides