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Literature summary extracted from

  • Han, Q.; Calvo, E.; Marinotti, O.; Fang, J.; Rizzi, M.; James, A.A.; Li, J.
    Analysis of the wild-type and mutant genes encoding the enzyme kynurenine monooxygenase of the yellow fever mosquito, Aedes aegypti (2003), Insect Mol. Biol., 12, 483-490.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.14.13.9 gene kh, DNA and amino acid sequence analysis of wild-type and mutant genes, expression in Spodoptera frugiperda Sf9 cells as His-tagged, soluble protein via the baculovirus infection system Aedes aegypti

Protein Variants

EC Number Protein Variants Comment Organism
1.14.13.9 additional information an inactive mutant lacks 162 nucleotides near the 3'-end of the mutant allele, the in-frame deletion results in loss of 54 amino acids leading to loss of enzyme activity and white eyes Aedes aegypti

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.14.13.9 chloride mixed-type inhibition Aedes aegypti
1.14.13.9 pyridoxal 5'-phosphate noncompetitive Aedes aegypti

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.14.13.9 additional information
-
 additional information kinetics Aedes aegypti
1.14.13.9 0.82
-
 NADPH recombinant enzyme, pH 7.5, 37°C Aedes aegypti
1.14.13.9 0.89
-
 L-kynurenine recombinant enzyme, pH 7.5, 37°C Aedes aegypti
1.14.13.9 5.17
-
 NADH recombinant enzyme, pH 7.5, 37°C Aedes aegypti

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.14.13.9 membrane enzyme is hydrophobis and contains 2 transmembrane segments Aedes aegypti 16020
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.14.13.9 54000
-
 x * 54000, recombinant soluble enzyme not counting the His-tag, SDS-PAGE Aedes aegypti

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.14.13.9 L-kynurenine + NADPH + O2 Aedes aegypti enzyme has a key role in L-tryptophan catabolism and in synthesis of ommochrome pigments in the eyes of the mosquitos 3-hydroxy-L-kynurenine + NADP+ + H2O
-
 ?
1.14.13.9 L-kynurenine + NADPH + O2 Aedes aegypti Liverpool enzyme has a key role in L-tryptophan catabolism and in synthesis of ommochrome pigments in the eyes of the mosquitos 3-hydroxy-L-kynurenine + NADP+ + H2O
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.14.13.9 Aedes aegypti Q86PM2 yellow fever mosquito, black-eyed Liverpool strain
-
1.14.13.9 Aedes aegypti Liverpool Q86PM2 yellow fever mosquito, black-eyed Liverpool strain
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.14.13.9 recombinant His-tagged enzyme from soluble fraction of Sf9 insect cells by nickel affinity chromatography Aedes aegypti

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.13.9 L-kynurenine + NADH + H+ + O2
-
 Aedes aegypti 3-hydroxy-L-kynurenine + NAD+ + H2O
-
 ?
1.14.13.9 L-kynurenine + NADH + H+ + O2
-
 Aedes aegypti Liverpool 3-hydroxy-L-kynurenine + NAD+ + H2O
-
 ?
1.14.13.9 L-kynurenine + NADPH + O2
-
 Aedes aegypti 3-hydroxy-L-kynurenine + NADP+ + H2O
-
 ?
1.14.13.9 L-kynurenine + NADPH + O2 enzyme has a key role in L-tryptophan catabolism and in synthesis of ommochrome pigments in the eyes of the mosquitos Aedes aegypti 3-hydroxy-L-kynurenine + NADP+ + H2O
-
 ?
1.14.13.9 L-kynurenine + NADPH + O2
-
 Aedes aegypti Liverpool 3-hydroxy-L-kynurenine + NADP+ + H2O
-
 ?
1.14.13.9 L-kynurenine + NADPH + O2 enzyme has a key role in L-tryptophan catabolism and in synthesis of ommochrome pigments in the eyes of the mosquitos Aedes aegypti Liverpool 3-hydroxy-L-kynurenine + NADP+ + H2O
-
 ?

Subunits

EC Number Subunits Comment Organism
1.14.13.9 ? x * 54000, recombinant soluble enzyme not counting the His-tag, SDS-PAGE Aedes aegypti

Synonyms

EC Number Synonyms Comment Organism
1.14.13.9 KMO
-
 Aedes aegypti
1.14.13.9 kynurenine hydroxylase
-
 Aedes aegypti
1.14.13.9 kynurenine monooxygenase
-
 Aedes aegypti
1.14.13.9 More the enzyme is a member of the glutathione reductase structural family Aedes aegypti

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.14.13.9 40
-
-
 Aedes aegypti

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.14.13.9 0.85
-
 NADH recombinant enzyme, pH 7.5, 37°C Aedes aegypti
1.14.13.9 1.88
-
 L-kynurenine recombinant enzyme, pH 7.5, 37°C Aedes aegypti
1.14.13.9 2.03
-
 NADPH recombinant enzyme, pH 7.5, 37°C Aedes aegypti

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.14.13.9 7.5
-
-
 Aedes aegypti

Cofactor

EC Number Cofactor Comment Organism Structure
1.14.13.9 FAD consensus domain sequence, probably FAD-containing Aedes aegypti
1.14.13.9 NADH low activity, ineffective cofactor Aedes aegypti
1.14.13.9 NADPH highly preferred cofactor with respect to NADH Aedes aegypti

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
1.14.13.9 0.17
-
 pyridoxal 5'-phosphate recombinant enzyme, with respect to NADPH, pH 7.5, 37°C Aedes aegypti
1.14.13.9 0.27
-
 pyridoxal 5'-phosphate recombinant enzyme, with respect to L-kynurenine, pH 7.5, 37°C Aedes aegypti
1.14.13.9 11.2
-
 chloride recombinant enzyme, with respect to L-kynurenine, pH 7.5, 37°C Aedes aegypti
1.14.13.9 24.5
-
 chloride recombinant enzyme, with respect to NADPH, pH 7.5, 37°C Aedes aegypti