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Literature summary extracted from

  • Champreda, V.; Zhou, N.Y.; Leak, D.J.
    Heterologous expression of alkene monooxygenase components from Xanthobacter autotrophicus Py2 and reconstitution of the active complex (2004), FEMS Microbiol. Lett., 239, 309-318.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.14.13.69 native and His-tagged protein expressed in Escherichia coli Rosetta(DE3)(pLysS) Xanthobacter autotrophicus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.14.13.69 Iron non-haem iron Xanthobacter autotrophicus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.14.13.69 9650
-
 alpha2beta2gamma2, 2 * 58100, 2 * 38000, 2 * 9650, calculated from deduced amino acid sequences, masses in SDS-PAGE appear to be smaller (alpha and gamma subunits) or larger (beta subunit) Xanthobacter autotrophicus
1.14.13.69 38000
-
 alpha2beta2gamma2, 2 * 58100, 2 * 38000, 2 * 9650, calculated from deduced amino acid sequences, masses in SDS-PAGE appear to be smaller (alpha and gamma subunits) or larger (beta subunit) Xanthobacter autotrophicus
1.14.13.69 58100
-
 alpha2beta2gamma2, 2 * 58100, 2 * 38000, 2 * 9650, calculated from deduced amino acid sequences, masses in SDS-PAGE appear to be smaller (alpha and gamma subunits) or larger (beta subunit) Xanthobacter autotrophicus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.14.13.69 propene + NADH + O2 Xanthobacter autotrophicus
-
 1,2-epoxypropane + NAD+ + H2O
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.14.13.69 Xanthobacter autotrophicus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.14.13.69 recombinant proteins from Escherichia coli Xanthobacter autotrophicus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.13.69 propene + NADH + O2
-
 Xanthobacter autotrophicus 1,2-epoxypropane + NAD+ + H2O
-
 ?

Subunits

EC Number Subunits Comment Organism
1.14.13.69 hexamer alpha2beta2gamma2, 2 * 58100, 2 * 38000, 2 * 9650, calculated from deduced amino acid sequences, masses in SDS-PAGE appear to be smaller (alpha and gamma subunits) or larger (beta subunit) Xanthobacter autotrophicus

Synonyms

EC Number Synonyms Comment Organism
1.14.13.69 More part of a multicomponent enzyme complex Xanthobacter autotrophicus

Cofactor

EC Number Cofactor Comment Organism Structure
1.14.13.69 NADH
-
 Xanthobacter autotrophicus