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Literature summary extracted from

  • de Paula, R.M.; Wilson, W.A.; Roach, P.J.; Terenzi, H.F.; Bertolini, M.C.
    Biochemical characterization of Neurospora crassa glycogenin (GNN), the self-glucosylating initiator of glycogen synthesis (2005), FEBS Lett., 579, 2208-2214.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.4.1.186 expression in Escherichia coli as a GST-tagged protein Neurospora crassa

Protein Variants

EC Number Protein Variants Comment Organism
2.4.1.186 DELTA306-664 7fold increase in self-glucosylation Neurospora crassa
2.4.1.186 DELTA306-664/Y196F no self-glucosylation activity Neurospora crassa
2.4.1.186 DELTA306-664/Y196F/Y198F expression results in no accumulation of glycogen Neurospora crassa
2.4.1.186 DELTA360-664 11.8fold increase in self-glucosylation Neurospora crassa
2.4.1.186 DELTA360-664/Y196F expression results in reduced glycogen accumulation to 30% of the wild-type enzyme, very low self-glucosylation activity Neurospora crassa
2.4.1.186 DELTA360-664/Y196F/Y198F no self-glucosylation activity Neurospora crassa

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.4.1.186 0.00441
-
 UDP-glucose
-
 Neurospora crassa

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.4.1.186 UDP-glucose + glycogenin Neurospora crassa self-glucosylating initiator of glycogen synthesis UDP + glucosylglycogenin
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.4.1.186 Neurospora crassa Q6Q2C8
-
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
2.4.1.186 glycoprotein two glucosylation sites Tyr196 and Tyr198. The enzyme is a self-glucosylating initiator of glycogen synthesis, recombinant enzyme contains an oligosaccharide chain attached to Tyr196 Neurospora crassa

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.1.186 UDP-glucose + glycogenin
-
 Neurospora crassa UDP + glucosylglycogenin
-
 ?
2.4.1.186 UDP-glucose + glycogenin self-glucosylating initiator of glycogen synthesis Neurospora crassa UDP + glucosylglycogenin
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.4.1.186 GNN
-
 Neurospora crassa